Bacteriophages as factories for Eu2O3 nanoparticle synthesis

Piotr Golec , Kamila Żelechowska , Joanna Karczewska-Golec , Jakub Karczewski , Adam Leśniewski , Marcin Łoś , Grzegorz Węgrzyn , Andrzej Marian Kłonkowski

Abstract

The use of phage display to identify peptides with an ability to bind and synthesize Eu2O3 nanoparticles is demonstrated in this report. This is the first report of modified phages specifically binding a lanthanide. The peptides exposed on virions revealed very strong binding to Eu2O3 nanoparticles and the ability to catalyze Eu2O3 nanoparticles’ formation from Eu(OH)3 and Eu(NO3)3 solutions. The luminescence emission spectrum of Eu3+ ions indicated that these ions existed mostly in sites deviated from the inversion symmetry in crystalline Eu2O3 aggregates and gelatinous Eu(OH)3 precipitate. The ability of phage-displayed peptides to catalyze formation of Eu2O3 nanoparticles provides a useful tool for a low-cost and effective synthesis of lanthanide nanoparticles, which serve as attractive biomedical sensors or fluorescent labels, among their other applications.
Author Piotr Golec
Piotr Golec,,
-
, Kamila Żelechowska
Kamila Żelechowska,,
-
, Joanna Karczewska-Golec (FB / DMGB)
Joanna Karczewska-Golec,,
- Department of Molecular Genetics of Bacteria
, Jakub Karczewski
Jakub Karczewski,,
-
, Adam Leśniewski
Adam Leśniewski,,
-
, Marcin Łoś (FB / DMGB)
Marcin Łoś,,
- Department of Molecular Genetics of Bacteria
, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Andrzej Marian Kłonkowski (FCh)
Andrzej Marian Kłonkowski,,
- Faculty of Chemistry
Journal seriesBioconjugate Chemistry, ISSN 1043-1802, (A 35 pkt)
Issue year2017
Vol28
No7
Pages1834-1841
Publication size in sheets0.5
DOIDOI:10.1021/acs.bioconjchem.7b00119
URL http://pubs.acs.org/doi/pdf/10.1021/acs.bioconjchem.7b00119
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.818 (2) - 2016=4.574 (5)
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