Biodiversity analysis of the unique geothermal microbial ecosystem of the Blue Lagoon (Iceland) using next- generation sequencing (NGS)

Katarzyna Palińska , J. C. Vogt , Waldemar Surosz

Abstract

The Blue Lagoon is a shallow geothermal lake which contains sea- and freshwater and is extremely high in silica content. This study has provided the first in-depth molecular survey of its planktic microbial inhabitants and laid the ground-work for future environmental and physiological work. Most MiSeq sequences obtained were affiliated with Alphaproteobacteria (36%), Cyanobacteria(26%), and Gammaproteobacteria (24%). Cyanobacteria were found to be the principal primary producers with 99.8% of the cyanobacterial sequences belonging to the unicellularCyanobacterium aponinum. Filamentous genera: Halomicronemaand Phormidium were very minor and present only outside the bathing area. The calculated diversity was typical of samples from extreme environments. Elevated salinity andhigh concentration of silicium attract extremophiles that are either highly adapted specialists for certain conditions or generalists with wide tolerance ranges. Relatively few abundant phyla were represented; 95% of observed OTUs consisted of less than ten sequences and only 0.5% had more than 1000 sequences. All rarefaction curves were far from saturation and a higher number of sequences is apparently required to cover the whole community diversity. Although, the diversity of the abundant taxa was limited, the rarebiosphere, detectable only with next generationsequencing, increased significantly the diversity.
Author Katarzyna Palińska (FOG / IO / DMBE)
Katarzyna Palińska,,
- Department of Marine Biology and Ecology
, J. C. Vogt
J. C. Vogt,,
-
, Waldemar Surosz (FOG / IO / DMBE)
Waldemar Surosz,,
- Department of Marine Biology and Ecology
Journal seriesHydrobiologia, ISSN 0018-8158, (A 30 pkt)
Issue year2018
Vol811
No1
Pages93-102
Publication size in sheets0.5
Keywords in EnglishReykjanes, halophilic, amplicon sequencing, Cyanobacterium aponinum, extremophiles
DOIDOI:10.1007/s10750-017-3349-2
URL https://link.springer.com/content/pdf/10.1007%2Fs10750-017-3349-2.pdf
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 19-04-2018, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 19-04-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.056 (2) - 2016=2.447 (5)
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