The domain swapping of human cystatin C induced by synchrotron radiation

Michal Taube , Zuzanna Pietralik , Aneta Szymańska , Kosma Szutkowski , Daniel Clemens , Anders Grubb , Maciej Kozak

Abstract

Domain swapping is observed for many proteins with flexible conformations. This phenomenon is often associated with the development of conformational diseases. Importantly, domain swapping has been observed for human cystatin C (HCC), a protein capable of forming amyloid deposits in brain arteries. In this study, the ability of short exposure to high-intensity X-ray radiation to induce domain swapping in solutions of several HCC variants (wild-type HCC and V57G, V57D, V57N, V57P, and L68V mutants) was determined. The study was conducted using time-resolved small-angle X-ray scattering (TR-SAXS) synchrotron radiation. The protein samples were also analysed using small-angle neutron scattering and NMR diffusometry. Exposing HCC to synchrotron radiation (over 50 ms) led to a gradual increase in the dimeric fraction, and for exposures longer than 150 ms, the oligomer fraction was dominant. In contrast, the non-irradiated protein solutions, apart from the V57P variant, were predominantly monomeric (e.g., V57G) or in monomer/dimer equilibrium. This work might represent the first observation of domain swapping induced by high-intensity X-rays.
Author Michal Taube - [Uniwersytet im. Adama Mickiewicza w Poznaniu]
Michal Taube,,
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- Uniwersytet im. Adama Mickiewicza w Poznaniu
, Zuzanna Pietralik - [Uniwersytet im. Adama Mickiewicza w Poznaniu]
Zuzanna Pietralik,,
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- Uniwersytet im. Adama Mickiewicza w Poznaniu
, Aneta Szymańska (FCh / DBCh / LMCh)
Aneta Szymańska,,
- Laboratory of Medical Chemistry
, Kosma Szutkowski - [Uniwersytet im. Adama Mickiewicza w Poznaniu]
Kosma Szutkowski,,
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- Uniwersytet im. Adama Mickiewicza w Poznaniu
, Daniel Clemens - [Helmholtz-Zentrum Berlin für Materialien und Energie (HZB)]
Daniel Clemens,,
-
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, Anders Grubb - [Skånes universitetssjukhus]
Anders Grubb,,
-
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, Maciej Kozak - [Uniwersytet im. Adama Mickiewicza w Poznaniu]
Maciej Kozak,,
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- Uniwersytet im. Adama Mickiewicza w Poznaniu
Journal seriesScientific Reports, ISSN 2045-2322, (N/A 140 pkt)
Issue year2019
Vol9
Pages1-11
Publication size in sheets0.5
Article number8548
ASJC Classification1000 Multidisciplinary
DOIDOI:10.1038/s41598-019-44811-1
URL https://doi.org/10.1038/s41598-019-44811-1
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 03-04-2020, ArticleFromJournal
Publication indicators Scopus Citations = 3; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.401; WoS Impact Factor: 2018 = 4.011 (2) - 2018=4.525 (5)
Citation count*5 (2020-01-04)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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