The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
Aleksandra Dydecka , Sylwia Bloch , Agnieszka Necel , Gracja Topka , Alicja Węgrzyn , Jinge Tong , Logan W. Donaldson , Grzegorz Węgrzyn , Bożena Nejman-Faleńczyk
AbstractThe exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein.
|Journal series||Virus Genes, ISSN 0920-8569, e-ISSN 1572-994X, (N/A 70 pkt)|
|Keywords in English||Exo-xis region, lambdoid phages development, Shiga toxin-producing Escherichia coli (STEC)|
|ASJC Classification||; ; ;|
|License||Other; published final; ; with publication|
|Score||= 70.0, 22-05-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2016 = 0.685; : 2018 = 1.616 (2) - 2018=1.478 (5)|
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