The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity

Aleksandra Dydecka , Sylwia Bloch , Agnieszka Necel , Gracja Topka , Alicja Węgrzyn , Jinge Tong , Logan W. Donaldson , Grzegorz Węgrzyn , Bożena Nejman-Faleńczyk


The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein.
Publication typeIn press (online first, early view)
Author Aleksandra Dydecka (FB / DMoB)
Aleksandra Dydecka ,,
- Department of Molecular Biology
, Sylwia Bloch - [Polish Academy of Science]
Sylwia Bloch,,
- Polska Akademia Nauk
, Agnieszka Necel (FB / DMoB)
Agnieszka Necel,,
- Department of Molecular Biology
, Gracja Topka (FB / DMoB)
Gracja Topka,,
- Department of Molecular Biology
, Alicja Węgrzyn - [Institute of Biochemistry and Biophysics of the Polish Academy of Sciences]
Alicja Węgrzyn,,
- Institute of Biochemistry and Biophysics of the Polish Academy of Sciences
, Jinge Tong - [York University]
Jinge Tong,,
, Logan W. Donaldson - [York University]
Logan W. Donaldson,,
, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
, Bożena Nejman-Faleńczyk (FB / DMoB)
Bożena Nejman-Faleńczyk,,
- Department of Molecular Biology
Journal seriesVirus Genes, ISSN 0920-8569, e-ISSN 1572-994X, (N/A 70 pkt)
Issue year2020
Noonline first
Keywords in EnglishExo-xis region, lambdoid phages development, Shiga toxin-producing Escherichia coli (STEC)
ASJC Classification2700 General Medicine; 1311 Genetics; 1312 Molecular Biology; 2406 Virology
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 22-05-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.685; WoS Impact Factor: 2018 = 1.616 (2) - 2018=1.478 (5)
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