High hydrogen peroxide production and antioxidative enzymes expression in the Chlamydomonas reinhardtii cia3 mutant with an increased tolerance to cadmium and anthracene

Anna Aksmann , Wojciech Pokora , Agnieszka Baścik-Remisiewicz , Agnieszka Dettlaff-Pokora , Zbigniew Tukaj

Abstract

Chlamydomonas reinhardtii cia3 mutant is deficient in activity of CAH3, a carbonic anhydrase isoform necessary for proper functioning of photosystem II (PS II). We used cia3 to verify the hypothesis that despite high concentration of external CO2, deficiency in CAH3 activity results in slight disorder of photosynthesis, which leads to mild but permanent overproduction of reactive oxygen species (ROS). As a consequence, cia3 should possess high ability to rebalance ROS homeostasis to overcome the oxidative stress induced by environmental factors, including chemical contaminations. We have found that in control conditions cia3 had a larger fraction of active PS II reaction centers but their efficiency was lower as compared with the wild type (WT), while H2O2 production by cia3 was significantly higher than in WT. In regard to ROS- scavenging enzymes, the transcript level for superoxide dis- mutase (SOD) isoforms and the isoenzymes activity in cia3 were higher than in WT. There were no significant differences in the transcript level of ascorbate peroxidase (APX) and catalase (CAT) genes in both strains, and slightly elevated activity of the enzymes was observed in the cia3 -cells. Comparing the sensitivity of WT and cia3 to chemically-induced stress we have found that the growth, photosynthesis and H2O2 production in WT were more strongly affected by cadmium and anthracene as compared with cia3 . SODs expression and activity in the treated WT were higher than those in cia3, but APX and CAT activities were similar in both strains. Our results indicate that the CAH3-deficiency is associated with markedly elevated H2O2 production and with a relatively higher initial transcript level and the activity of ROS- scavenging enzymes. In consequence, cia3 revealed a higher tolerance to cadmium and anthracene compared with WT.
Author Anna Aksmann (FB / DPPB)
Anna Aksmann,,
- Department of Plant Physiology and Biotechnology
, Wojciech Pokora (FB / DPPB)
Wojciech Pokora,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Baścik-Remisiewicz (FB / DPPB)
Agnieszka Baścik-Remisiewicz,,
- Department of Plant Physiology and Biotechnology
, Agnieszka Dettlaff-Pokora
Agnieszka Dettlaff-Pokora,,
-
, Zbigniew Tukaj (FB / DPPB)
Zbigniew Tukaj,,
- Department of Plant Physiology and Biotechnology
Journal seriesPhycological Research, ISSN 1322-0829, (A 20 pkt)
Issue year2016
Vol64
No4
Pages300-311
Publication size in sheets0.55
Keywords in Englishascorbate peroxidas, CAH3 deficiency, catalase, chemical stress, green algae, reactive oxygen species, superoxide dismutase
DOIDOI:10.1111/pre.12147
URL http://onlinelibrary.wiley.com/doi/10.1111/pre.12147/pdf
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 20.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.338 (2) - 2016=1.335 (5)
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