Effects of some commonly used drinks on induction of Shiga toxin-converting prophage in Escherichia coli
Karolina Pierzynowska , Weronika Jasińska , Zuzanna Cyske , Magdalena Bunikowska , Robert Droczek , Grzegorz Węgrzyn
AbstractPathogenicity of enterohemorrhagicEscherichia coli(EHEC) strains depends on various virulence factors, like adhesins(including intimin), flagella, type I fimbriae, curli fibers, hemolysins, and agents required for biofilm formation. However,the pathogenicity is highly enhanced by production of Shiga toxins which are encoded bystxgenes located in genomes ofShiga toxin-converting (Stx) prophages. Thus, for maximal EHEC virulence, induction of these prophages is necessary.Although various physical and chemical agents causing Stx prophage induction are known, it is not clear whether any foodcan stimulate this process. Therefore, the aim of this work was to test whether commonly used drinks might enhance Stxprophage induction inE. coli. It was found that 5% Nestea, but not 5% Coca-Cola or 1% ethanol, caused a significantinduction of 933W Stx prophage in a bacterial culture in vitro. These results indicate that some commonly used drinks canstimulate Stx prophage induction, potentially enhancing virulence of EHEC. This may have impact on protection ofconsumers which are infected by these bacteria, and might contribute to choose safe food for such persons.
|Journal series||Journal fur Verbraucherschutz und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety, ISSN 1661-5751, (A 15 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||enterohemorrhagic Escherichia coli, Shiga toxin-converting bacteriophages, commonly used drinks, prophage induction|
|License||Other; published final; ; with publication|
|Score|| = 15.0, ArticleFromJournal|
= 15.0, ArticleFromJournal
|Publication indicators||: 2017 = 0.581 (2) - 2017=0.726 (5); = 2; = 2|
|Citation count*||2 (2019-10-16)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.