Effects of some commonly used drinks on induction of Shiga toxin-converting prophage in Escherichia coli

Karolina Pierzynowska , Weronika Jasińska , Zuzanna Cyske , Magdalena Bunikowska , Robert Droczek , Grzegorz Węgrzyn


Pathogenicity of enterohemorrhagicEscherichia coli(EHEC) strains depends on various virulence factors, like adhesins(including intimin), flagella, type I fimbriae, curli fibers, hemolysins, and agents required for biofilm formation. However,the pathogenicity is highly enhanced by production of Shiga toxins which are encoded bystxgenes located in genomes ofShiga toxin-converting (Stx) prophages. Thus, for maximal EHEC virulence, induction of these prophages is necessary.Although various physical and chemical agents causing Stx prophage induction are known, it is not clear whether any foodcan stimulate this process. Therefore, the aim of this work was to test whether commonly used drinks might enhance Stxprophage induction inE. coli. It was found that 5% Nestea, but not 5% Coca-Cola or 1% ethanol, caused a significantinduction of 933W Stx prophage in a bacterial culture in vitro. These results indicate that some commonly used drinks canstimulate Stx prophage induction, potentially enhancing virulence of EHEC. This may have impact on protection ofconsumers which are infected by these bacteria, and might contribute to choose safe food for such persons.
Author Karolina Pierzynowska (FB / DMoB)
Karolina Pierzynowska,,
- Department of Molecular Biology
, Weronika Jasińska (FB)
Weronika Jasińska,,
- Faculty of Biology
, Zuzanna Cyske (FB)
Zuzanna Cyske,,
- Faculty of Biology
, Magdalena Bunikowska (FB)
Magdalena Bunikowska,,
- Faculty of Biology
, Robert Droczek (FB)
Robert Droczek,,
- Faculty of Biology
, Grzegorz Węgrzyn (FB / DMoB)
Grzegorz Węgrzyn,,
- Department of Molecular Biology
Journal seriesJournal fur Verbraucherschutz und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety, ISSN 1661-5751, (A 15 pkt)
Issue year2018
Publication size in sheets0.5
Keywords in Englishenterohemorrhagic Escherichia coli, Shiga toxin-converting bacteriophages, commonly used drinks, prophage induction
URL https://doi.org/10.1007/s00003-018-1155-z
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)15
ScoreMinisterial score = 15.0, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2017 = 0.581 (2) - 2017=0.726 (5); Scopus Citations = 2; WoS Citations = 2
Citation count*2 (2019-10-16)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.