Behavioral and physiological effects of Viola spp. cyclotides on Myzus persicae (Sulz.)

Katarzyna Dancewicz , Błażej Ślązak , Małgorzata Kiełkiewicz , Małgorzata Kapusta , Jerzy Bohdanowicz , Beata Gabryś

Abstract

Cyclotides are defense peptides produced by several plant families. Viola spp. (Violaceae) produce an array of cyclotides with varying biological activities. The peach potato aphid Myzus persicae (Sulz.) (Hemiptera: Aphididae) is a generalist that feeds on the secondary hosts of over 40 plant families, including Violaceae. The present work aimed to evaluate the activities of cycloviolacins from Viola odorata L. and V. ulignosa Besser (cyO2, cyO3, cyO13, cyO19) against M. persicae. To investigate the peptides’ influence on aphid feeding behavior, we used 20% sucrose diets supplemented with cyclotides and measured the effects with electrical penetration graph (EPG) technique. We also applied anti-cyclotide antibodies and immunohistochemistry to track the peptides in the digestive systems of the aphids. Our study shows that cyclotides affect aphid probing and feeding behavior and limit their diet sap uptake. The cycloviolacin cyclotides: cyO13 (100 µM) and cyO19 (50 µM) most strongly impeded aphid ingestion activities when applied in sucrose diet. Sustained ingestion of the diet was blocked by 100 µM cyO13, and no aphid showed ingestion of the diet for longer than 10 min. Cyclotides were detected in the pharynx, in contact with the epipharyngeal gustatory organ, in the stomach (midgut) and upper intestine. The present study shows the deterrent activity of cycloviolacins on M. persicae. This activity may be related to the peptides’ effects on epithelial cells and gustatory organs along the aphid digestive system. We demonstrate that cyclotides may play an important role in plant-aphid interactions.
Author Katarzyna Dancewicz
Katarzyna Dancewicz,,
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, Błażej Ślązak
Błażej Ślązak,,
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, Małgorzata Kiełkiewicz
Małgorzata Kiełkiewicz,,
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, Małgorzata Kapusta (FB / DPCE)
Małgorzata Kapusta,,
- Department of Plant Cytology and Embryology
, Jerzy Bohdanowicz (FB / DPCE)
Jerzy Bohdanowicz,,
- Department of Plant Cytology and Embryology
, Beata Gabryś
Beata Gabryś,,
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Journal seriesJournal of Insect Physiology, ISSN 0022-1910, e-ISSN 1879-1611, (N/A 100 pkt)
Issue year2020
Vol122
Pages1-12
Publication size in sheets0.55
Article number104025
Keywords in EnglishCyclic peptides, plant defense, electrical penetration graph (EPG), immunohistochemistry, defensive peptides, aphid-plant interactions
ASJC Classification1109 Insect Science; 1314 Physiology
DOIDOI:10.1016/j.jinsphys.2020.104025
URL https://doi.org/10.1016/j.jinsphys.2020.104025
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 18-05-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.025; WoS Impact Factor: 2018 = 2.862 (2) - 2018=2.768 (5)
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