Method for detection of hydrogen peroxide in HT22 cells
Dagmara Jacewicz , Kamila Siedlecka-Kroplewska , Joanna Drzeżdżon , Agnieszka Piotrowska , Dariusz Wyrzykowski , Aleksandra Tesmar , Krzysztof Żamojć , Lech Chmurzyński
AbstractWe have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C2O4)(pm)(OH2)2]+ (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.
|Journal series||Scientific Reports, ISSN 2045-2322, (A 40 pkt)|
|Publication size in sheets||0.5|
|License||Journal (articles only); published final; ; with publication|
|Score|| = 40.0, 20-12-2017, ArticleFromJournal|
= 40.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2016 = 4.259 (2) - 2016=4.847 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.