Method for detection of hydrogen peroxide in HT22 cells

Dagmara Jacewicz , Kamila Siedlecka-Kroplewska , Joanna Drzeżdżon , Agnieszka Piotrowska , Dariusz Wyrzykowski , Aleksandra Tesmar , Krzysztof Żamojć , Lech Chmurzyński

Abstract

We have proposed a new method which can be applied in assessing the intracellular production of hydrogen peroxide. Using this assay we have examined the hydrogen peroxide generation during the L-glutamate induced oxidative stress in the HT22 hippocampal cells. The detection of hydrogen peroxide is based on two crucial reagents cis-[Cr(C2O4)(pm)(OH2)2]+ (pm denotes pyridoxamine) and 2-ketobutyrate. The results obtained indicate that the presented method can be a promising tool to detect hydrogen peroxide in biological samples, particularly in cellular experimental models.
Author Dagmara Jacewicz (FCh / DGICh / LPCC)
Dagmara Jacewicz,,
- Laboratory of Physicochemistry of Coordination Complexes
, Kamila Siedlecka-Kroplewska
Kamila Siedlecka-Kroplewska,,
-
, Joanna Drzeżdżon (FCh / DGICh / LPCC)
Joanna Drzeżdżon,,
- Laboratory of Physicochemistry of Coordination Complexes
, Agnieszka Piotrowska (FCh / DGICh / LPCC)
Agnieszka Piotrowska,,
- Laboratory of Physicochemistry of Coordination Complexes
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Aleksandra Tesmar (FCh / DGICh / LPCC)
Aleksandra Tesmar,,
- Laboratory of Physicochemistry of Coordination Complexes
, Krzysztof Żamojć (FCh / DGICh / LPCC)
Krzysztof Żamojć,,
- Laboratory of Physicochemistry of Coordination Complexes
, Lech Chmurzyński (FCh / DGICh / LPCC)
Lech Chmurzyński,,
- Laboratory of Physicochemistry of Coordination Complexes
Journal seriesScientific Reports, ISSN 2045-2322, (A 40 pkt)
Issue year2017
Vol7
Pages1-6
Publication size in sheets0.5
DOIDOI:10.1038/srep45673
URL https://www.nature.com/articles/srep45673
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.259 (2) - 2016=4.847 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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