Increased expression of the leptin gene in adipose tissue of patients with chronic kidney disease - the possible role of an abnormal serum fatty acid profile

Justyna Korczyńska , Aleksandra Czumaj , Michał Chmielewski , Maciej Śledziński , Adriana Mika , Tomasz Śledziński

Abstract

Chronic kidney disease (CKD) is associated with an increased level of leptin and an abnormal fatty acid (FA) profile in the serum. However, there are no data on the associations between them, and the reason for increased serum levels in patients with CKD is not well elucidated. Recently, we found that a CKD-related abnormal FA profile caused significant changes in the expression of genes involved in lipid metabolism in hepatocytes. The aim of this study was to examine whether leptin gene expression in subcutaneous adipose tissue (SAT) of patients with CKD may contribute to increased serum levels of this adipokine and whether the abnormal serum FA profile observed in CKD patients has an impact on leptin gene expression in adipocytes. The FA profile was measured in serum samples from patients with CKD and controls by GC–MS. The relative mRNA levels of leptin were measured in SAT by Real-Time PCR. Moreover, the effect of the CKD-related abnormal FA profile on leptin gene expression was studied in in vitro cultured 3T3-L1 adipocytes. Patients with CKD had higher concentrations of serum leptin than controls and higher expression level of the leptin gene in SAT. They also had increased serum monounsaturated FAs and decreased polyunsaturated FAs. The incubation of adipocytes with FAs isolated from CKD patients resulted in an increase of the levels of leptin mRNA. Increased leptin gene expression in SAT may contribute to elevated concentrations of these adipokine in patients with CKD. CKD-related alterations of the FA profile may contribute to elevated serum leptin concentrations in patients with CKD by increasing the gene expression of this adipokine in SAT.
Author Justyna Korczyńska
Justyna Korczyńska,,
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, Aleksandra Czumaj
Aleksandra Czumaj,,
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, Michał Chmielewski
Michał Chmielewski,,
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, Maciej Śledziński
Maciej Śledziński,,
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, Adriana Mika (FCh / DEA / LChER)
Adriana Mika,,
- Laboratory of Chemical Environmental Risks
, Tomasz Śledziński
Tomasz Śledziński,,
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Journal seriesMetabolites, ISSN 2218-1989, (N/A 70 pkt)
Issue year2020
Vol10
No3
Pages1-11
Publication size in sheets0.5
Article number98
Keywords in Englishchronic kidney disease, leptin, fatty acids, adipose tissue, adipocytes
ASJC Classification2712 Endocrinology, Diabetes and Metabolism; 1303 Biochemistry; 1312 Molecular Biology
DOIDOI:10.3390/metabo10030098
Languageen angielski
LicenseRepository; published final; Uznanie Autorstwa (CC-BY); with publication
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Korczynska_Justyna_Increased_expression_2020.pdf 1.62 MB
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 09-03-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.124; WoS Impact Factor: 2018 = 3.303 (2)
Citation count*
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LicencjaUtwór jest udostępniany na licencji Creative Commons Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/d
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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