Application of silver nanostructures synthesized by cold atmospheric pressure plasma for inactivation of bacterial phytopathogens from the genera "Dickeya" and "Pectobacterium"

Anna Dzimitrowicz , Agata Motyka , Piotr Jamroz , Ewa Łojkowska , Weronika Babińska , Dominik Terefinko , Paweł Pohl , Wojciech Śledź

Abstract

Pectinolytic bacteria are responsible for significant economic losses by causing diseases on numerous plants. New methods are required to control and limit their spread. One possibility is the application of silver nanoparticles (AgNPs) that exhibit well-established antibacterial properties. Here, we synthesized AgNPs, stabilized by pectins (PEC) or sodium dodecyl sulphate (SDS), using a direct current atmospheric pressure glow discharge (dc-APGD) generated in an open-to-air and continuous-flow reaction-discharge system. Characterization of the PEC-AgNPs and SDS-AgNPs with UV/Vis absorption spectroscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, and selected area electron diffraction revealed the production of spherical, well dispersed, and face cubic centered crystalline AgNPs, with average sizes of 9.33 ± 3.37 nm and 28.3 ± 11.7 nm, respectively. Attenuated total reflection-Fourier transformation infrared spectroscopy supported the functionalization of the nanostructures by PEC and SDS. Antibacterial activity of the AgNPs was tested against Dickeya spp. and Pectobacterium spp. strains. Both PEC-AgNPs and SDS-AgNPs displayed bactericidal activity against all of the tested isolates, with minimum inhibitory concentrations of 5.5 mg∙L−1 and 0.75–3 mg∙L−1, respectively. The collected results suggest that the dc-APGD reaction-discharge system can be applied for the production of defined AgNPs with strong antibacterial properties, which may be further applied in plant disease management.
Author Anna Dzimitrowicz
Anna Dzimitrowicz,,
-
, Agata Motyka MWB UG i GUMed
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Piotr Jamroz
Piotr Jamroz,,
-
, Ewa Łojkowska KB
Ewa Łojkowska,,
- Department of Biotechnology
, Weronika Babińska MWB UG i GUMed
Weronika Babińska,,
- Intercollegiate Faculty of Biotechnology UG
, Dominik Terefinko
Dominik Terefinko,,
-
, Paweł Pohl
Paweł Pohl,,
-
, Wojciech Śledź KB
Wojciech Śledź,,
- Department of Biotechnology
Journal seriesMaterials, ISSN 1996-1944
Issue year2018
Vol11
No3
Pages1-16
Publication size in sheets0.75
Keywords in Englishnanoparticles, atmospheric pressure glow discharge, plasma-liquid interactions, pectinolytic bacteria, plant protection, soft rot, blackleg
DOIDOI:10.3390/ma11030331
URL http://www.mdpi.com/1996-1944/11/3/331/pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)35
ScoreMinisterial score = 35.0, 30-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 30-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.654 (2) - 2016=3.236 (5)
Citation count*0
Cite
Share Share

Get link to the record
msginfo.png


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back