Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/ spoT mutant : bioprocess characterization and transcriptome analysis

Justyna Mozejko-Ciesielska , Dorota Dabrowska , Agnieszka Szalewska-Pałasz , Sławomir Ciesielski

Abstract

Pseudomonas putida KT2440 is a model bacteria used commonly for medium chain length polyhydroxyalkanoates (mcl PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl PHAs synthesis in reaction to environmental stimuli are still not clear. The rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes in order to promote survival under nutritional deprivation conditions. Therefore, in this work we investigated the relation between mcl-PHAs synthesis and stringent response. For this study, a relA/spot mutant of P. putida KT2440, unable to induce the stringent response, was used. Additionally, the transcriptome of this mutant was analyzed using RNA seq in order to examine rearrangements of the metabolism during cultivation. The results show that the relA/spoTmutant of P. putida KT2440 is able to accumulate mcl PHAs in both optimal and nitrogen limiting conditions. Nitrogen starvation did not change the efficiency of mcl PHAs synthesis in this mutant. The transition from exponential growth to stationary phase caused significant upregulation of genes involved in transport system and nitrogen metabolism. Transcriptional regulators, including rpoS, rpoN and rpoD, did not show changes in transcript abundance when entering the stationary phase, suggesting their limited role in mcl PHAs accumulation during stationary phase.
Author Justyna Mozejko-Ciesielska
Justyna Mozejko-Ciesielska,,
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, Dorota Dabrowska
Dorota Dabrowska,,
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, Agnieszka Szalewska-Pałasz (FB / DMGB)
Agnieszka Szalewska-Pałasz,,
- Department of Molecular Genetics of Bacteria
, Sławomir Ciesielski
Sławomir Ciesielski,,
-
Journal seriesAMB Express, ISSN 2191-0855, (A 25 pkt)
Issue year2017
Vol7
No1
Pages1-13
Publication size in sheets0.6
Keywords in Englishglobal regulation, Pseudomonas putida KT2440, polyhydroxyalkanoates, stringent response, transcriptomics
DOIDOI:10.1186/s13568-017-0396-z
URL https://amb-express.springeropen.com/track/pdf/10.1186/s13568-017-0396-z?site=amb-express.springeropen.com
Languageen angielski
LicenseJournal (articles only); author's final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)25
ScoreMinisterial score = 25.0, 15-01-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 15-01-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.825 (2) - 2016=2.332 (5)
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