Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds
Sylwia Bloch , Bożena Nejman-Faleńczyk , Karolina Pierzynowska , Ewa Piotrowska , Alicja Węgrzyn , Christelle Marminon , Zouhair Bouaziz , Pascal Nebois , Joachim Jose , Marc Le Borgne , Luciano Saso , Grzegorz Węgrzyn
AbstractOxidative stress may be the major cause of induction of Shiga toxin-converting (Stx) prophages from chromosomes of Shiga toxin-producing Escherichia coli (STEC) in human intestine. Thus, we aimed to test a series of novel antioxidant compounds for their activities against prophage induction, thus, preventing pathogenicity of STEC. Forty-six compounds (derivatives of carbazole, indazole, triazole, quinolone, ninhydrine, and indenoindole) were tested. Fifteen of them gave promising results and were further characterized. Eleven compounds had acceptable profiles in cytotoxicity tests with human HEK-293 and HDFa cell lines. Three of them (selected for molecular studies) prevent the prophage induction at the level of expres- sion of specific phage genes. In bacterial cells treated with hydrogen peroxide, expression of genes involved in the oxidative stress response was significantly less efficient in the presence of the tested compounds. Therefore, they apparently reduce the oxidative stress, which prevents induction of Stx prophage in E. coli.
|Journal series||Journal of Enzyme Inhibition and Medicinal Chemistry, ISSN 1475-6366, (A 25 pkt)|
|Publication size in sheets||0.55|
|Keywords in English||Shiga toxin-producing, Escherichia coli, Shiga toxin-converting bacteriophage, oxidative stress, antioxidants, heterocyclic compounds|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 25.0, 29-11-2019, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 1.067; : 2018 = 4.027 (2) - 2018=3.181 (5)|
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