Arthrospiribacter ruber gen. nov., sp. nov., a novel bacterium isolated from Arthrospira cultures

Małgorzata Waleron , Agnieszka Misztak , Michał Waleron , Magda A. Furmaniak , Agnieszka Mrozik , Krzysztof Waleron

Abstract

Polyphasic analysis of ten isolates of the red-pigmented bacteria isolated from ten Arthrospira cultures originating from different parts of the world is described. The 16S rRNA analysis showed <95 % identity with the known bacteria on public databases, therefore, additional analyses of fatty acids profiles, MALDI-TOF/MS, genome sequencing of the chosen isolate and following phylogenomic analyses were performed. Gram-stain-negative, strictly aerobic rods were positive for catalase, negative for oxidase, proteolytic and urease activity. Major fatty acids were 15 : 0 iso, 17:0 iso 3 OH and 17:1 iso w9c/16:0 10-methyl. The whole phylogenomic analyses revealed that the genomic sequence of newly isolated strain DPMB0001 was most closely related to members of Cyclobacteriaceae family and clearly indicated distinctiveness of newly isolated bacteria. The average nucleotide identity and in silico DNA–DNA hybridisation values were calculated between representative of the novel strains DPMB0001 and its phylogenetically closest species, Indibacter alkaliphilus CCUG57479 (LW1)T (ANI 69.2 % is DDH 17.2 %) and Mariniradius saccharolyticus AK6T (ANI 80.02 % isDDH 26.1 %), and were significantly below the established cut-off <94 % (ANI) and <70 % (isDDH) for species and genus delineation. The obtained results showed that the analysed isolates represent novel genus and species, for which names Arthrospiribacter gen nov. and Arthrospiribacter ruber sp. nov. (type strain DPMB0001 = LMG 31078 = PCM 3008) is proposed.
Author Małgorzata Waleron (IFB / IB / DBiotech)
Małgorzata Waleron,,
- Department of Biotechnology
, Agnieszka Misztak (IFB)
Agnieszka Misztak,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Waleron (IFB) - [Medical Uniwersity of Gdańsk (GUMed)]
Michał Waleron,,
- Intercollegiate Faculty of Biotechnology UG
- Gdański Uniwersytet Medyczny
, Magda A. Furmaniak
Magda A. Furmaniak,,
-
, Agnieszka Mrozik
Agnieszka Mrozik,,
-
, Krzysztof Waleron - [Medical Uniwersity of Gdańsk (GUMed)]
Krzysztof Waleron,,
-
- Gdański Uniwersytet Medyczny
Journal seriesSystematic and Applied Microbiology, ISSN 0723-2020, (N/A 100 pkt)
Issue year2020
Vol43
No2
Pages1-9
Publication size in sheets0.5
Article number126072
Keywords in EnglishECE, low-income, workforce, classroom, quality
ASJC Classification1105 Ecology, Evolution, Behavior and Systematics; 2402 Applied Microbiology and Biotechnology; 2404 Microbiology
DOIDOI:10.1016/j.syapm.2020.126072
URL https://doi.org/10.1016/j.syapm.2020.126072
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 11-03-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.368; WoS Impact Factor: 2018 = 2.808 (2) - 2018=3.64 (5)
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