Effect of omiganan on colonic anastomosis healing in a rat model of peritonitis

Teresa Lorenzi , Maria Michela Cappelletti Trombettoni , Roberto Ghiselli , Francesca Paolinelli , Rosaria Gesuita , Oskar Cirioni , Mauro Provinciali , Wojciech Kamysz , Elżbieta Kamysz , Cristiano Piangatelli , Mario Castellucci , Mario Guerrieri , Manrico Morroni

Abstract

Background: This study investigates the effects of the antimicrobial cationic peptide omiganan-alone and combined with the antibiotic imipenem-on colonic anastomosis healing in presence of intraperitoneal sepsis induced in a rodent model of cecal ligation and puncture (CLP). Methods: Forty male Wistar rats were divided into 5 groups of 8 animals. Group 1 (control group) underwent laparotomy and cecal mobilization and the next day received left colon anastomosis. In group 2 (CLP without treatment), group 3(CLP + imipenem), group 4 (CLP + omiganan), and group 5 (CLP + omiganan + imipenem), the left colon anastomosis was performed the day after CLP. Imipenem and omiganan were administered by intraperitoneal injection immediately before anastomosis construction and subsequently at 24 h intervals until the 7th postoperative day, when rats were sacrificed. Anastomotic bursting pressure was measured in situ. Tissue samples were collected for determination of hydroxyproline content and histological characteristics.Results: Only rats receiving omiganan + imipenem displayed re-epithelialization, reduced neovascularization of granulation tissue, and a bursting pressure that was similar to that of controls. Omiganan-alone and combined with imipenem-was associated with a better control of inflammatory parameters than imipenem alone. In addition omiganan, like imipenem, counteracted the collagen depletion typical of sepsis.Conclusions: This experimental study demonstrates the efficacy of the new antimicrobial agent omiganan, alone and in combination with imipenem, in delaying the effects of intraperitoneal sepsis on colonic anastomosis healing and provides evidence of the value of omiganan as a therapeutic agent.
Author Teresa Lorenzi
Teresa Lorenzi,,
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, Maria Michela Cappelletti Trombettoni
Maria Michela Cappelletti Trombettoni,,
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, Roberto Ghiselli
Roberto Ghiselli,,
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, Francesca Paolinelli
Francesca Paolinelli,,
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, Rosaria Gesuita
Rosaria Gesuita,,
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, Oskar Cirioni
Oskar Cirioni,,
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, Mauro Provinciali
Mauro Provinciali,,
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, Wojciech Kamysz
Wojciech Kamysz,,
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, Elżbieta Kamysz PChMB
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Cristiano Piangatelli
Cristiano Piangatelli,,
-
et al.`
Other language title versions
Journal seriesAmerican Journal of Translational Research, ISSN 1943-8141
Issue year2017
Vol9
No7
Pages3374-3386
Publication size in sheets0.6
Keywords in Englishrat model of peritonitis, colonic anastomosis, omiganan, wound healing process, bursting pressure, hydroxyproline concentration
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553886/pdf/ajtr0009-3374.pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne (CC-BY-NC); with publication
Score (nominal)35
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.829 (2) - 2016=3.011 (5)
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