Extracellular HtrA serine proteases: an emerging new strategy in bacterial pathogenesis

Steffen Backert , Sabine Bernegger , Joanna Skórko-Glonek , Silja Wessler

Abstract

The HtrA family of chaperones and serine proteases is important for regulating stress responses and controlling protein quality in the periplasm of bacteria. HtrA is also associated with infectious diseases since inactivation of htrA genes results in significantly reduced virulence properties by various bacterial pathogens. These virulence features of HtrA can be attributed to reduced fitness of the bacteria, higher susceptibility to environmental stress and/or diminished secretion of virulence factors. In some Gram‐negative and Gram‐positive pathogens, HtrA itself can be exposed to the extracellular environment promoting bacterial colonisation and invasion of host tissues. Most of our knowledge on the function of exported HtrAs stems from research on Helicobacter pylori, Campylobacter jejuni, Borrelia burgdorferi, Bacillus anthracis, and Chlamydia species. Here, we discuss recent progress showing that extracellular HtrAs are able to cleave cell‐to‐cell junction factors including E‐cadherin, occludin, and claudin‐8, as well as extracellular matrix proteins such as fibronectin, aggrecan, and proteoglycans, disrupting the epithelial barrier and producing substantial host cell damage. We propose that the export of HtrAs is a newly discovered strategy, also applied by additional bacterial pathogens. Consequently, exported HtrA proteases represent highly attractive targets for antibacterial treatment by inhibiting their proteolytic activity or application in vaccine development.
Author Steffen Backert
Steffen Backert,,
-
, Sabine Bernegger
Sabine Bernegger,,
-
, Joanna Skórko-Glonek (FB / DB)
Joanna Skórko-Glonek,,
- Department of Biochemistry
, Silja Wessler
Silja Wessler,,
-
Journal seriesCellular Microbiology, ISSN 1462-5814, (A 35 pkt)
Issue year2018
Vol20
No6
Pages1-9
Publication size in sheets0.5
Keywords in Englishadherens junction, aggrecan, chaperone, claudin, E‐cadherin, fibronectin, HtrA, infection biology, occludin, OMV, outer membrane vesicles, proteoglycan, secretion, serine protease, tight junction
DOIDOI:10.1111/cmi.12845
URL https://doi.org/10.1111/cmi.12845
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 35.0, 29-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 29-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.554 (2) - 2016=4.579 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back