Molecular, morphological and ultrastructural characteristics of Prasiola crispa (Lightfoot) Kützing (Chlorophyta) from Spitsbergen (Arctic)

Dorota Richter , Jan Matuła , Jacek Urbaniak , Małgorzata Waleron , Joanna Czerwik-Marcinkowska

Abstract

It is commonly known that seabirds, along with cyanobacteria, play a key role in the enrichment of nutrient-poor polar terrestrial ecosystems. In habitats particularly enriched with nitrogen and phosphate ions, ornithogenic vegetation with Prasiola crispa and unique species composition develops. However, no studies have examined the potentially different impacts associated with seabirds on the morphological, ultrastructural and molecular diversity of P. crispa. For that purpose, the present study analyzed four populations from the Hornsund Fjord area (West Spitsbergen) collected in the vicinity of planktivorous or piscivorous colonies and individual nests of seabirds. The morphological variability of P. crispa was studied using fresh samples, and it was documented with light microscopy as well as transmission and scanning electron microscopy. Populations were investigated by molecular analyses based on rbcL and 18S rRNA sequences. The differences in features measured were subjected to statistical analysis. This study provides new data concerning the macro- and submicroscopic structure and molecular diversity of P. crispa with regard to differences in humidity and the quantity of biogenic nutrients deposited by seabirds. Molecular research provided the first data about P. crispa from Spitsbergen. Birds’ diet is relevant for the quantity and quality of the provided nutrients, especially nitrogen compounds (planktivorous species) and phosphorus (piscivorous birds). Climate changes influence the quantitative structure of the colony, clearly favoring piscivorous birds. This, in turn, causes changes in the environment and vegetation. The obtained data on the P. crispa population may be compared with potential changes in the future.
Author Dorota Richter
Dorota Richter,,
-
, Jan Matuła
Jan Matuła,,
-
, Jacek Urbaniak
Jacek Urbaniak,,
-
, Małgorzata Waleron (IFB / IB / DBiotech)
Małgorzata Waleron,,
- Department of Biotechnology
, Joanna Czerwik-Marcinkowska
Joanna Czerwik-Marcinkowska,,
-
Journal seriesPolar Biology, ISSN 0722-4060, (A 30 pkt)
Issue year2017
Vol40
No2
Pages379-397
Publication size in sheets0.9
Keywords in EnglishPrasiola crispa, variation of Svalbard strains, influence of seabirds, rbcL, 18S rRNA genes
DOIDOI:10.1007/s00300-016-1966-2
URL http://link.springer.com/content/pdf/10.1007%2Fs00300-016-1966-2.pdf
Languageen angielski
Score (nominal)30
ScoreMinisterial score = 30.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.949 (2) - 2016=2.066 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back