Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review

Robert Czajkowski , M. C. M. Pérombelon , Sylwia Jafra , Ewa Łojkowska , Marta Potrykus , J. M. van der Wolf , Wojciech Śledź


The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost‐effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in seed certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less‐investigated ones.
Author Robert Czajkowski (IFB / IB / DBiotech)
Robert Czajkowski,,
- Department of Biotechnology
, M. C. M. Pérombelon
M. C. M. Pérombelon,,
, Sylwia Jafra (IFB / IB / DBiotech)
Sylwia Jafra,,
- Department of Biotechnology
, Ewa Łojkowska (IFB / IB / DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
, Marta Potrykus (IFB / IB / DBiotech)
Marta Potrykus,,
- Department of Biotechnology
, J. M. van der Wolf
J. M. van der Wolf,,
, Wojciech Śledź (IFB / IB / DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
Journal seriesAnnals of Applied Biology, ISSN 0003-4746, (A 40 pkt)
Issue year2015
Publication size in sheets1
Keywords in EnglishBiovar, isolation, molecular techniques, PCR, pectinolytic Erwinia spp., phylogeny, potato, serology
ASJC Classification1102 Agronomy and Crop Science
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, ArticleFromJournal
Publication indicators WoS Citations = 36; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.172; WoS Impact Factor: 2015 = 2.103 (2) - 2015=2.062 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.