Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review
Robert Czajkowski , M. C. M. Pérombelon , Sylwia Jafra , Ewa Łojkowska , Marta Potrykus , J. M. van der Wolf , Wojciech Śledź
AbstractThe soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost‐effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in seed certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less‐investigated ones.
|Journal series||Annals of Applied Biology, ISSN 0003-4746, (A 35 pkt)|
|Publication size in sheets||1|
|Keywords in English||Biovar, isolation, molecular techniques, PCR, pectinolytic Erwinia spp., phylogeny, potato, serology|
|License||Other; published final; ; with publication|
|Score|| = 40.0, 20-12-2017, ArticleFromJournal|
= 40.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2015 = 2.103 (2) - 2015=2.062 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.