Farnesol potentiates photodynamic inactivation of Staphylococcus aureus with the use of red light-activated porphyrin TMPyP

Monika Kossakowska-Zwierucho , Grzegorz Szewczyk , Tadeusz Sarna , Joanna Nakonieczna

Abstract

Photodynamic inactivation (PDI) or antibacterial photodynamic therapy (aPDT) is a method based on the use of a photosensitizer, light of a proper wavelength and oxygen, which combined together leads to an oxidative stress and killing of target cells. PDI can be applied towards various pathogenic bacteria independently on their antibiotic resistance profile. Optimization of photodynamic treatment to eradicate the widest range of human pathogens remains challenging despite the availability of numerous photosensitizing compounds. Therefore, a search for molecules that could act as adjuvants potentiating antibacterial photoinactivation is of high scientific and clinical importance. Here we propose farnesol (FRN), a well described sesquiterpene, as a potent adjuvant of PDI, which specifically sensitizes Staphylococcus aureus to 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetratosylate (TMPyP) upon red light irradiation. Interestingly, the observed potentiation strongly depends on the presence of light. Analysis of this combined action of FRN and TMPyP, however, showed no influence of farnesol on TMPyP photochemical properties, i.e. the amount of reactive oxygen species that were produced by TMPyP in the presence of FRN. The accumulation rate of TMPyP in Staphylococcus aureus cells did not change, as well as the influence of staphyloxanthin inhibition. The precise mechanism of observed sensitization is unclear and probably involves specific molecular targets.
Author Monika Kossakowska-Zwierucho (IFB)
Monika Kossakowska-Zwierucho,,
- Intercollegiate Faculty of Biotechnology UG
, Grzegorz Szewczyk
Grzegorz Szewczyk,,
-
, Tadeusz Sarna
Tadeusz Sarna,,
-
, Joanna Nakonieczna (IFB/IB/DBiotech)
Joanna Nakonieczna,,
- Department of Biotechnology
Journal seriesJournal of Photochemistry and Photobiology B-Biology, [Journal of Photochemistry and Photobiology B: Biology], ISSN 1011-1344, (N/A 100 pkt)
Issue year2020
Vol206
Pages1-10
Publication size in sheets0.50
Article number111863
Keywords in EnglishPhotodynamic inactivation, Staphylococcus aureus, membrane fluidity, reactive oxygen species detection
ASJC Classification2741 Radiology Nuclear Medicine and imaging; 3614 Radiological and Ultrasound Technology; 1304 Biophysics; 3108 Radiation
DOIDOI:10.1016/j.jphotobiol.2020.111863
URL https://doi.org/10.1016/j.jphotobiol.2020.111863
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 17-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.078; WoS Impact Factor: 2018 = 4.067 (2) - 2018=3.721 (5)
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