Fecal serine protease profiling in inflammatory bowel diseases

Amin Jablaoui , Aicha Kriaa , Héla Mkaouar , Nizar Akermi , Souha Soussou , Magdalena Wysocka , Dominika Wołoszyn , Ali Amouri , Ali Gargouri , Emmanuelle Maguin , Adam Lesner , Moez Rhimi

Abstract

Serine proteases are extensively known to play key roles in many physiological processes. However, their dysregulation is often associated to several diseases including inflammatory bowel diseases (IBD). Here, we used specific substrates to monitor fecal protease activities in a large cohort of healthy and IBD patients. Of interest, serine protease activity was 10-fold higher in IBD fecal samples compared to healthy controls. Moreover, functional analysis of these fecal proteolytic activities revealed that the most increased activities are trypsin-like, elastase-like and cathepsin G-like. We also show for the first time, an increase of proteinase 3-like activity in these samples compared to controls. Results presented here will guide further investigations to better understand the relevance of these peptidases in IBD.
Author Amin Jablaoui
Amin Jablaoui,,
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, Aicha Kriaa
Aicha Kriaa,,
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, Héla Mkaouar
Héla Mkaouar,,
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, Nizar Akermi
Nizar Akermi,,
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, Souha Soussou
Souha Soussou,,
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, Magdalena Wysocka (FCh / DBCh / LMCh)
Magdalena Wysocka,,
- Laboratory of Medical Chemistry
, Dominika Wołoszyn (FCh / DET / LBAN)
Dominika Wołoszyn,,
- Laboratory of Biochemical Analytics and Nanodiagnostics
, Ali Amouri
Ali Amouri,,
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, Ali Gargouri
Ali Gargouri,,
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, Emmanuelle Maguin
Emmanuelle Maguin,,
-
et al.`
Journal seriesFrontiers in cellular and infection microbiology, ISSN 2235-2988, (N/A 100 pkt)
Issue year2020
Vol10
Pages1-7
Publication size in sheets0.5
Article number21
Keywords in Englishfecal proteases, inflammatory bowel diseases, gut microbiota, protease profiling, serine protease inhibitors, Holobiont, microbiome
ASJC Classification2725 Infectious Diseases; 2726 Microbiology (medical); 2403 Immunology; 2404 Microbiology
DOIDOI:10.3389/fcimb.2020.00021
URL https://www.frontiersin.org/articles/10.3389/fcimb.2020.00021/full
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 07-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.005; WoS Impact Factor: 2018 = 3.518 (2) - 2018=4.42 (5)
Citation count*1 (2020-03-31)
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