Qualitative and quantitative analysis of proteome and peptidome of human follicular fluid using multiple samples from single donor with LC-MS and SWATH methodology

Aleksandra Lewandowska , Katarzyna Macur , Paulina Czaplewska , Joanna Liss , Krzysztof Łukaszuk , Stanisław Ołdziej

Abstract

Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small-scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient-specific. Ultrafiltration was used to fractionate hFF to high-molecular-weight (HMW) proteome (>10 kDa) and low-molecular-weight (LMW) peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC–MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, from which 59 were never reported before as hFF components. 55 (45 not reported before) proteins were found by analyzing LMW fraction, 67 (14 not reported before) were found by analyzing HMW fraction, and 36 were identified in both fractions of hFF. We were able to perform quantitative analysis for 72 proteins from HMW fraction of hFF. We found that concentrations of 11 proteins varied substantially among hFF samples from single donors, and those proteins are promising targets to identify biomarkers useful in oocyte quality assessment.
Autor Aleksandra Lewandowska (MWB UG i GUMed)
Aleksandra Lewandowska
- Międzyuczelniany Wydział Biotechnologii UG i GUMed
, Katarzyna Macur (MWB UG i GUMed / ZLS)
Katarzyna Macur
- Zespół Laboratoriów Specjalistycznych MWB UG i GUMed
, Paulina Czaplewska (MWB UG i GUMed / M020 / KBMiK)
Paulina Czaplewska
- Katedra Biologii Molekularnej i Komórkowej
, Joanna Liss
Joanna Liss
-
, Krzysztof Łukaszuk
Krzysztof Łukaszuk
-
, Stanisław Ołdziej (MWB UG i GUMed / IB UG / PSB)
Stanisław Ołdziej
- Pracownia Struktury Biopolimerów
Inne wersje tytułu
Tytuł czasopisma/seriiJournal of Proteome Research, ISSN 1535-3893, (A 35 pkt)
Rok wydania2017
Tom16
Nr8
Paginacja3053-3067
Objętość publikacji w arkuszach wydawniczych0.7
Słowa kluczowe w języku angielskimhuman follicular fluid, LC-MS, oocyte quality control, peptidome, proteome, SWATH
Klasyfikacja ASJC1600 General Chemistry; 1303 Biochemistry
DOIDOI:10.1021/acs.jproteome.7b00366
URL http://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.7b00366
Języken angielski
Punktacja (całkowita)35
PunktacjaPunktacja MNiSW = 35.0, 24-07-2019, ArticleFromJournal
Wskaźniki publikacji Cytowania WoS = 5; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.982; Impact Factor WoS: 2017 = 3.95 (2) - 2017=4.033 (5)
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