Qualitative and quantitative analysis of proteome and peptidome of human follicular fluid using multiple samples from single donor with LC-MS and SWATH methodology

Aleksandra Lewandowska , Katarzyna Macur , Paulina Czaplewska , Joanna Liss , Krzysztof Łukaszuk , Stanisław Ołdziej

Abstract

Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small-scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient-specific. Ultrafiltration was used to fractionate hFF to high-molecular-weight (HMW) proteome (>10 kDa) and low-molecular-weight (LMW) peptidome (<10 kDa) fractions. HMW and LMW compositions were analyzed using LC–MS in SWATH data acquisition and processing methodology. In total we were able to identify 158 proteins, from which 59 were never reported before as hFF components. 55 (45 not reported before) proteins were found by analyzing LMW fraction, 67 (14 not reported before) were found by analyzing HMW fraction, and 36 were identified in both fractions of hFF. We were able to perform quantitative analysis for 72 proteins from HMW fraction of hFF. We found that concentrations of 11 proteins varied substantially among hFF samples from single donors, and those proteins are promising targets to identify biomarkers useful in oocyte quality assessment.
Author Aleksandra Lewandowska MWB UG i GUMed
Aleksandra Lewandowska,,
- Intercollegiate Faculty of Biotechnology UG
, Katarzyna Macur ZLS
Katarzyna Macur,,
- Zespół Laboratoriów Specjalistycznych MWB UG i GUMed
, Paulina Czaplewska KBMiK
Paulina Czaplewska,,
- Department of Molecular and Cellular Biology
, Joanna Liss
Joanna Liss,,
-
, Krzysztof Łukaszuk
Krzysztof Łukaszuk,,
-
, Stanisław Ołdziej PSB
Stanisław Ołdziej,,
- Laboratory of Biopolymers Structure
Other language title versions
Journal seriesJournal of Proteome Research, ISSN 1535-3893
Issue year2017
Vol16
No8
Pages3053-3067
Publication size in sheets0.7
Keywords in Englishhuman follicular fluid, LC-MS, oocyte quality control, peptidome, proteome, SWATH
DOIDOI:10.1021/acs.jproteome.7b00366
URL http://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.7b00366
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.268 (2) - 2016=4.43 (5)
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