Baculovirus expression and potential diagnostic application of the gp51 envelope glycoprotein of genetic mutants of the bovine leukaemia virus

Marzena Rola-Łuszczak , Agnieszka Grabowska , Bogusław Szewczyk , Jacek Kuźmak

Abstract

Introduction: Field isolates of bovine leukaemia virus (BLV) show the presence of a few amino acid substitutions in major conformational G and H epitopes on surface glycoprotein gp51. Potentially, these substitutions can affect the 3D structure of these epitopes leading to their diminished immunoreactivity. The aim of this study was to express three gp51 glycoproteins carrying mutated epitopes as recombinant baculovirus proteins in insect cells to test their immunoreactivity with bovine sera. Material and Methods: Env gene chimeras encoding mutated epitopes G and H in the env backbone of BLV FLK strain were constructed, cloned into pFastBac1 vector, and expressed in baculovirus. Results: The presence of recombinant gp51 protein in Sf9 insect cells was confirmed using monoclonal antibodies. ELISA tests were developed to check the immunoreactivity of recombinant protein with bovine sera. Conclusion: Recombinant gp51 proteins with altered G and H epitopes can be used for further studies to analyse the serological response of bovine sera towards BLV antigenic variants.
Author Marzena Rola-Łuszczak
Marzena Rola-Łuszczak,,
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, Agnieszka Grabowska (IFB)
Agnieszka Grabowska,,
- Intercollegiate Faculty of Biotechnology UG
, Bogusław Szewczyk (IFB / IB / LRV)
Bogusław Szewczyk,,
- Laboratory of Recombinant Vaccines
, Jacek Kuźmak
Jacek Kuźmak,,
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Journal seriesJournal of Veterinary Research [Bulletin of the Veterinary Institute in Pulawy], ISSN 2450-7393, e-ISSN 2450-8608, (A 15 pkt)
Issue year2019
Vol63
No1
Pages1-6
Publication size in sheets0.5
Keywords in Englishbovine leukaemia virus, conformational epitopes, recombinant baculovirus protein
ASJC Classification3400 General Veterinary
DOIDOI:10.2478/jvetres-2019-0020
URL https://doi.org/10.2478/jvetres-2019-0020
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Score (nominal)15
ScoreMinisterial score = 15.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.364; WoS Impact Factor: 2017 = 0.811 (2) - 2017=0.973 (5)
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