Application of HS-SPME-GC-MS for the analysis of aldehydes produced by different insect species and their antifungal activity

Aleksandra Bojke , Cezary Tkaczuk , Marta Bauer , Wojciech Kamysz , Marek Gołębiowski

Abstract

In this study, a procedure was developed to determine aldehydes using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography (GC) coupled with mass spectrometry (MS). The aldehydes selected for research had previously been identified in various species of insects. Minimal inhibitory concentrations of the compounds against strains of entomopathogenic fungi were also determined. At the outset, the best SPME extraction conditions were chosen for the analysis to obtain good chromatographic separation. The analysis was carried out using a BZ-5 column and different SPME fibers were used to isolate the aldehydes. DVB/CAR/PDMS fiber appeared to be the most efficient coating for undertaking the measurements. The best parameters of separation by HS-SPME and analysis by GC–MS were selected. In addition, the aldehydes were tested for their potential antifungal activity. A procedure was developed to determine the aldehydes using HS-SPME-GC–MS. Heptanal, 2,4-nonadienal, 2-decenal and undecanal were the most effective antifungal compounds against entomopathogenic fungi.
Author Aleksandra Bojke (FCh / DEA / LANC)
Aleksandra Bojke,,
- Laboratory of Analysis of Natural Compounds
, Cezary Tkaczuk
Cezary Tkaczuk,,
-
, Marta Bauer
Marta Bauer,,
-
, Wojciech Kamysz
Wojciech Kamysz,,
-
, Marek Gołębiowski (FCh / DEA / LANC)
Marek Gołębiowski,,
- Laboratory of Analysis of Natural Compounds
Journal seriesJournal of Microbiological Methods, ISSN 0167-7012, e-ISSN 1872-8359, (N/A 70 pkt)
Issue year2020
Vol169
Pages1-7
Publication size in sheets0.5
Article number105835
Keywords in EnglishHS-SPME-GC-MS, aldehydes, MIC, entomopathogenic fungi, antifungal activity
ASJC Classification2726 Microbiology (medical); 1312 Molecular Biology; 2404 Microbiology
DOIDOI:10.1016/j.mimet.2020.105835
URL https://doi.org/10.1016/j.mimet.2020.105835
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.7; WoS Impact Factor: 2018 = 1.803 (2) - 2018=2.027 (5)
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