Modern methods of sample preparation for the analysis of oxylipins in biological samples

Ivan Liakh , Alicja Pakiet , Tomasz Śledziński , Adriana Mika


Oxylipins are potent lipid mediators derived from polyunsaturated fatty acids, which play important roles in various biological processes. Being important regulators and/or markers of a wide range of normal and pathological processes, oxylipins are becoming a popular subject of research; however, the low stability and often very low concentration of oxylipins in samples are a significant challenge for authors and continuous improvement is required in both the extraction and analysis techniques. In recent years, the study of oxylipins has been directly related to the development of new technological platforms based on mass spectrometry (LC–MS/MS and gas chromatography–mass spectrometry (GC–MS)/MS), as well as the improvement in methods for the extraction of oxylipins from biological samples. In this review, we systematize and compare information on sample preparation procedures, including solid-phase extraction, liquid–liquid extraction from different biological tissues.
Author Ivan Liakh
Ivan Liakh,,
, Alicja Pakiet (FCh / DEA / LChER)
Alicja Pakiet,,
- Laboratory of Chemical Environmental Risks
, Tomasz Śledziński
Tomasz Śledziński,,
, Adriana Mika (FCh / DEA / LChER)
Adriana Mika,,
- Laboratory of Chemical Environmental Risks
Journal seriesMolecules, ISSN 1420-3049, (N/A 100 pkt)
Issue year2019
Publication size in sheets1.8
Article number1639
Keywords in Englishsample preparation, oxylipins, protein precipitation, liquid–liquid extraction, solidphase extraction, biological samples
ASJC Classification1605 Organic Chemistry
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 17-11-2019, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.146; WoS Impact Factor: 2018 = 3.06 (2) - 2018=3.38 (5)
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