Modern methods of sample preparation for the analysis of oxylipins in biological samples
Ivan Liakh , Alicja Pakiet , Tomasz Śledziński , Adriana Mika
AbstractOxylipins are potent lipid mediators derived from polyunsaturated fatty acids, which play important roles in various biological processes. Being important regulators and/or markers of a wide range of normal and pathological processes, oxylipins are becoming a popular subject of research; however, the low stability and often very low concentration of oxylipins in samples are a significant challenge for authors and continuous improvement is required in both the extraction and analysis techniques. In recent years, the study of oxylipins has been directly related to the development of new technological platforms based on mass spectrometry (LC–MS/MS and gas chromatography–mass spectrometry (GC–MS)/MS), as well as the improvement in methods for the extraction of oxylipins from biological samples. In this review, we systematize and compare information on sample preparation procedures, including solid-phase extraction, liquid–liquid extraction from different biological tissues.
|Journal series||Molecules, ISSN 1420-3049, (A 30 pkt)|
|Publication size in sheets||1.8|
|Keywords in English||sample preparation, oxylipins, protein precipitation, liquid–liquid extraction, solidphase extraction, biological samples|
|License||Journal (articles only); published final; ; with publication|
|Score||= 30.0, 24-07-2019, ArticleFromJournal|
|Publication indicators||: 2017 = 1.146; : 2017 = 3.098 (2) - 2017=3.268 (5)|
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