The use of fosmid metagenomic libraries in preliminary screening for various biological activities

Agnieszka Felczykowska , Aleksandra Dydecka , Małgorzata Bohdanowicz , Tomasz Gąsior , Marek Soboň , Justyna Kobos , Sylwia Bloch , Bożena Nejman-Faleńczyk , Grzegorz Węgrzyn

Abstract

Background: It is generally believed that there are many natural sources of as yet unknown bioactive compounds with a high biotechnological potential. However, the common method based on the use of cell extracts in the preliminary screening for particular molecules or activities is problematic as amounts of obtained compounds may be low, and such experiments are hardly reproducible. Therefore, the aim of this work was to test whether a novel strategy to search for previously unknown biological activities can be efficient. This strategy is based on construction of metagenomic libraries and employment of Escherichia coli strains as cell factories producing compounds of properties potentially useful in biotechnology. Results: Three cyanobacterial metagenomic libraries were constructed in the fosmid system. The libraries were screened for various biological activities. Extracts from selected E. coli clones bearing constructs with fragments of cyanobacterial genomes revealed antimicrobial or anticancer activities. Interestingly, stimulation of growth of host bacteria bearing particular plasmids with certain cyanobacterial genes was detected, suggesting a potential possibility for improvement of E. coli cultivation during biotechnological production. The most interesting plasmids were sequenced, and putative mechanisms of biological effects caused by cyanobacterial gene products are discussed. Conclusions: The strategy of exploring cyanobacteria as sources of bioactive compounds, based on E. coli cell factories producing compounds due to expression of genes from metagenomic libraries, appears to be effective.
Author Agnieszka Felczykowska KBM
Agnieszka Felczykowska,,
- Department of Molecular Biology
, Aleksandra Dydecka KBM
Aleksandra Dydecka ,,
- Department of Molecular Biology
, Małgorzata Bohdanowicz KBM
Małgorzata Bohdanowicz ,,
- Department of Molecular Biology
, Tomasz Gąsior KBM
Tomasz Gąsior ,,
- Department of Molecular Biology
, Marek Soboň WB
Marek Soboň,,
- Faculty of Biology
, Justyna Kobos ZBM
Justyna Kobos,,
- Division of Marine Biotechnology
, Sylwia Bloch KBM
Sylwia Bloch,,
- Department of Molecular Biology
, Bożena Nejman-Faleńczyk KBM
Bożena Nejman-Faleńczyk,,
- Department of Molecular Biology
, Grzegorz Węgrzyn KBM
Grzegorz Węgrzyn,,
- Department of Molecular Biology
Journal seriesMicrobial Cell Factories, ISSN , e-ISSN 1475-2859
Issue year2014
Vol13
Pages1-7
Publication size in sheets0.5
Keywords in Englishmetagenomic libraries, cyanobacteria, Escherichia coli cell factories, bioactive compounds
DOIDOI:10.1186/s12934-014-0105-4
URL http://www.microbialcellfactories.com/content/pdf/s12934-014-0105-4.pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 40.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2014 = 4.221 (2) - 2014=4.642 (5)
Citation count*0
Cite
Share Share

Get link to the record
msginfo.png


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back