Silver nanoparticles combined with naphthoquinones as an effective synergistic strategy against Staphylococcus aureus

Marta Krychowiak , Anna Kawiak , Magdalena Narajczyk , Agnieszka Borowik , Aleksandra Królicka

Abstract

Staphylococcus aureus is a human pathogen responsible for many antibiotic-resistant infections, for instance burn wound infections, which pose a threat to human life. Exploring possible synergy between various antimicrobial agents, like nanoparticles and plant natural products, may provide new weapons to combat antibiotic resistant pathogens. The objective of this study was to examine the potential of silver nanoparticles (AgNPs) to enhance the antimicrobial activity of selected naphthoquinones (NQs): plumbagin (PL), ramentaceone (RAM), droserone (DR), and 3-chloroplumbagin (3ChPL). We also attempted to elucidate the mechanism by which the AgNPs enhance the antimicrobial activity of NQs. We analyzed the interaction of AgNPs with bacterial membrane and its effect on membrane stability (TEM analysis, staining with SYTO9 and propidium iodide), as well as aggregation of NQs on the surface of nanoparticles (UV-Vis spectroscopy and DLS analysis). Our results demonstrated clearly a synergistic activity of AgNPs and three out of four tested NQs (FBC indexes ≤ 0.375). This resulted in an increase in their combined bactericidal effect toward the S. aureus reference strain and the clinical isolates, which varied in resistance profiles. The synergistic effect (FBC index = 0.375) resulting from combining 3ChPL with silver nitrate used as a control, emphasized the role of the ionic form of silver released from nanoparticles in their bactericidal activity in combination with NQs. The role of membrane damage and AgNPs-NQ interactions in the observed synergy of silver nanoparticles and NQs was also confirmed. Moreover, the described approach, based on the synergistic interaction between the above mentioned agents enables a reduction of their effective doses, thus significantly reducing cytotoxic effect of NQs toward eukaryotic HaCaT cells. Therefore, the present study on the use of a combination of agents (AgNPs-NQs) suggests its potential use as a possible strategy to combat antibiotic-resistant S. aureus.
Autor Marta Krychowiak (MWB UG i GUMed)
Marta Krychowiak
- Międzyuczelniany Wydział Biotechnologii UG i GUMed
, Anna Kawiak (MWB UG i GUMed / IB UG / KB)
Anna Kawiak
- Katedra Biotechnologii
, Magdalena Narajczyk (WB / LME)
Magdalena Narajczyk
- Laboratorium Mikroskopii Elektronowej
, Agnieszka Borowik (MWB UG i GUMed)
Agnieszka Borowik
- Międzyuczelniany Wydział Biotechnologii UG i GUMed
, Aleksandra Królicka (MWB UG i GUMed / IB UG / KB)
Aleksandra Królicka
- Katedra Biotechnologii
Tytuł czasopisma/seriiFrontiers in Pharmacology, ISSN 1663-9812, (A 40 pkt)
Rok wydania2018
Tom9
Paginacja1-11
Objętość publikacji w arkuszach wydawniczych0.5
Słowa kluczowe w języku angielskimcombination of antimicrobials, Droseraceae, plant secondary metabolite, 1,4-naphthoquinone, antibiotic resistance, membrane damage, silver ions
Klasyfikacja ASJC2736 Pharmacology (medical); 3004 Pharmacology
DOIDOI:10.3389/fphar.2018.00816
URL https://www.frontiersin.org/articles/10.3389/fphar.2018.00816/pdf
Języken angielski
LicencjaCzasopismo (tylko dla artykułów); opublikowana ostateczna; Uznanie Autorstwa (CC-BY); w dniu opublikowania
Punktacja (całkowita)40
Żródło punktacjijournalList
PunktacjaPunktacja MNiSW = 40.0, 17-11-2019, ArticleFromJournal
Wskaźniki publikacji Cytowania WoS = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.017; Impact Factor WoS: 2018 = 3.845 (2) - 2018=4.469 (5)
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