Antimicrobial activity of different antimicrobial peptides (AMPs) against clinical methicillin-resistant Staphylococcus aureus (MRSA)

Eleonora Ciandrini , Gianluca Morroni , Daniela Arzeni , Wojciech Kamysz , Damian Neubauer , Elżbieta Kamysz , Oscar Cirioni , Lucia Brescini , Wally Baffone , Raffaella Campana

Abstract

Background: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool. Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates. Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies. Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 μg/ml), followed by Temporin A (4- 16 μg/ml), CA(1–7)M(2–9)NH2 (8-16 μg/ml) and Citropin 1.1 (16-64 μg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 μg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration. Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.
Author Eleonora Ciandrini
Eleonora Ciandrini,,
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, Gianluca Morroni
Gianluca Morroni,,
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, Daniela Arzeni
Daniela Arzeni,,
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, Wojciech Kamysz
Wojciech Kamysz,,
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, Damian Neubauer
Damian Neubauer,,
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, Elżbieta Kamysz (FCh / DMBt / LChBM)
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Oscar Cirioni
Oscar Cirioni,,
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, Lucia Brescini
Lucia Brescini,,
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, Wally Baffone
Wally Baffone,,
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, Raffaella Campana
Raffaella Campana,,
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Journal seriesCurrent Topics in Medicinal Chemistry, ISSN 1568-0266, (A 30 pkt)
Issue year2018
Vol18
No24
Pages2116-2126
Publication size in sheets0.5
Keywords in EnglishAMPs, methicillin-resistant Staphylococcus aureus, clinical isolates, antimicrobial activity, time-kill
DOIDOI:10.2174/1568026618666181022140348
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 30.0, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.561 (2) - 2016=2.848 (5)
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