Antimicrobial activity of different antimicrobial peptides (AMPs) against clinical methicillin-resistant Staphylococcus aureus (MRSA)
Eleonora Ciandrini , Gianluca Morroni , Daniela Arzeni , Wojciech Kamysz , Damian Neubauer , Elżbieta Kamysz , Oscar Cirioni , Lucia Brescini , Wally Baffone , Raffaella Campana
AbstractBackground: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool. Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates. Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies. Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 μg/ml), followed by Temporin A (4- 16 μg/ml), CA(1–7)M(2–9)NH2 (8-16 μg/ml) and Citropin 1.1 (16-64 μg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 μg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration. Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.
|Journal series||Current Topics in Medicinal Chemistry, ISSN 1568-0266, (A 30 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||AMPs, methicillin-resistant Staphylococcus aureus, clinical isolates, antimicrobial activity, time-kill|
|Score|| = 30.0, 10-12-2018, ArticleFromJournal|
= 40.0, 10-12-2018, ArticleFromJournal
|Publication indicators||: 2016 = 2.561 (2) - 2016=2.848 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.