Three-year pot culture of Epipactis helleborine reveals autotrophic survival, without mycorrhizal networks, in a mixotrophic species

Michał May , Marcin Jąkalski , Alzbeta Novotna , Jennifer Dietel , Manfred Ayasse , Felix Lallemand , Tomáš Figura , Julita Minasiewicz , Marc-Andre Selosse

Abstract

Some mixotrophic plants from temperate forests use the mycorrhizal fungi colonizing their roots as a carbon source to supplement their photosynthesis. These fungi are also mycorrhizal on surrounding trees, from which they transfer carbon to mixotrophic plants. These plants are thus reputed difficult to transplant, even when their protection requires it. Here, we take profit of a successful ex situ pot cultivation over 1 to 3 years of the mixotrophic orchid Epipacis helleborine to investigate its mycorrhizal and nutrition status. Firstly, compared with surrounding autotrophic plants, it did not display the higher N content and higher isotopic (13C and 15N) abundance that normally feature mixotrophic orchids because they incorporate N-, 13C-, and 15N-rich fungal biomass. Second, fungal barcoding by next-generation sequencing revealed that the proportion of ectomycorrhizal fungi (expressed as percentage of the total number of either reads or operational taxonomic units) was unusually low compared with E. helleborine growing in situ: instead, we found a high percentage of rhizoctonias, the usual mycorrhizal partners of autotrophic orchids. Altogether, this supports autotrophic survival. Added to the recently published evidence that plastid genomes of mixotrophic orchids have intact photosynthetic genes, this suggests that at least some of them have abilities for autotrophy. This adds to the ecological plasticity of mixotrophic plants, and may allow some reversion to autotrophy in their evolution.
Publication typeIn press (online first, early view)
Author Michał May (FB/DPTNC)
Michał May,,
- Department of Plant Taxonomy and Nature Conservation
, Marcin Jąkalski (FB/DPTNC)
Marcin Jąkalski,,
- Department of Plant Taxonomy and Nature Conservation
, Alzbeta Novotna (FB/DPTNC)
Alzbeta Novotna ,,
- Department of Plant Taxonomy and Nature Conservation
, Jennifer Dietel
Jennifer Dietel,,
-
, Manfred Ayasse
Manfred Ayasse,,
-
, Felix Lallemand
Felix Lallemand,,
-
, Tomáš Figura
Tomáš Figura,,
-
, Julita Minasiewicz (FB/DPTNC)
Julita Minasiewicz,,
- Department of Plant Taxonomy and Nature Conservation
, Marc-Andre Selosse (FB/DPTNC)
Marc-Andre Selosse,,
- Department of Plant Taxonomy and Nature Conservation
Journal seriesMycorrhiza, ISSN 0940-6360, e-ISSN 1432-1890, (N/A 100 pkt)
Issue year2020
Noonline first
Pages1-11
Publication size in sheets0.50
Keywords in Englishmycoheterotrophy, mycorrhizae, orchid transplantation, rhizoctonia, stable isotopes, 13C, 15N
ASJC Classification2700 General Medicine; 1105 Ecology, Evolution, Behavior and Systematics; 1110 Plant Science; 1311 Genetics; 1312 Molecular Biology
DOIDOI:10.1007/s00572-020-00932-4
Languageen angielski
LicenseRepository; published final; Uznanie Autorstwa (CC-BY); with publication
File
May_Michal_Three_year_pot_2020.pdf May_Michal_Three_year_pot_2020.pdf 746,85 KB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 09-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.186; WoS Impact Factor: 2018 = 3.114 (2) - 2018=3.349 (5)
Citation count*1 (2020-07-28)
Additional fields
LicencjaUtwór jest udostępniany na licencji Creative Commons Uznanie autorstwa 4.0 Międzynarodowe (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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