Peptydy penetrujące: rodzaje i mechanizmy przenikania przez błony komórkowe

Marcin T. Kawczyński , Joanna Kreczko-Kurzawa , Zbigniew Maćkiewicz

Abstract

Before discovery of the Tat peptide over twenty years ago, it was assumed that large peptides and proteins do not penetrate cell membranes. After discovery of Tat, Penetratin [1, 2] and several other peptides of natural origin, able to cross the cell membranes in an energy-independent manner, the structural determinants of their cell-penetrating potency were established, including polycationic character, amphi - paticity and presence of proline-rich motifs [3, 4]. C urrently known cell-penetrating peptides of natural or synthetic origin are composed of 5 to 40 amino acid residues and belong to one of the three families: oligocationic, amphipatic or proline-rich and penetrate the biological membranes by endocytosis or other ways of direct translocation. Most of these translocation mechanisms are not exclusive and may occur simultaneously, and their contribution may be different for each peptide depending on the conditions (e.g. CPP concentra - tion, pH, etc.). M ost CPPs demonstrate little or no mammalian cell toxicity what makes them promising vectors for drug delivery. Such vectors might be useful for efficient inter - nalization of compounds otherways poorly penetrating biological membranes. N ovel CPP-effector conjugates may become effective anticancer or antimicrobial agents of a great potential for chemotherapy [3, 5]. In this short review we present a glimpse at the current state of knowledge con - cerning sub - families, types and mechanisms of action of most prominent members o f CPP family.
Author Marcin T. Kawczyński
Marcin T. Kawczyński,,
-
, Joanna Kreczko-Kurzawa PChMB
Joanna Kreczko-Kurzawa,,
- Laboratory of Chemistry of Biological Macromolecules
, Zbigniew Maćkiewicz PChMB
Zbigniew Maćkiewicz,,
- Laboratory of Chemistry of Biological Macromolecules
Other language title versionsCell-penetrating peptides: types and mechanisms of penetration
Journal seriesWiadomości Chemiczne, ISSN 0043-5104, e-ISSN 2300-0295
Issue year2015
Vol69
No1-2
Pages9-33
Publication size in sheets1.2
Keywords in Polishpeptydy penetrujące, penetratyna, endocytoza, amfipatyczność
Keywords in EnglishCPP, cell-penetrating peptides, penetratin, endocytosis, amphipaticity
URL http://www.dbc.wroc.pl/dlibra/docmetadata?id=26571
Languagepl polski
LicenseOther; published final; Other open licence; with publication
Score (nominal)7
ScoreMinisterial score = 7.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 7.0, 28-11-2017, ArticleFromJournal
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