Alterations in the rainbow trout (Oncorhynchus mykiss) eggs exposed to ionizing radiation during induced androgenesis

Marcin Polonis , Krzysztof Jagiełło , Stefan Dobosz , Rafał Rożyński , Paulina Kondraciuk , Artur Gurgul , Tomasz Szmatoła , Konrad Ocalewicz

Abstract

Ionizing radiation (IR) is applied to inactivate nuclear genome in the salmonid eggs to induce androgenetic development. However, it has been considered that doses of IR used to damage maternal chromosomes may also affect morphology of the eggs and decrease their developmental potential. Thus, the main goal of the present research was to assess alterations in the rainbow trout (Oncorhynchus mykiss) eggs caused by the high dose of IR administered during androgenesis. In the present research, rainbow trout eggs were irradiated with 350 Gy of X‐rays, inseminated and exposed to the high hydrostatic pressure (HHP) shock to develop as androgenetic doubled haploids (DHs). The distribution of lipid droplets in the irradiated and non‐irradiated rainbow trout eggs, survival rates and morphology of larvae from androgenetic and control groups were compared. It has been observed that non‐irradiated and irradiated eggs exhibited altered distribution of lipid droplets. Most of the eggs before IR treatment displayed rather equal distribution of the oil droplets. In turn, majority of eggs studied after irradiation had coalesced lipid droplets, a pattern found in eggs with reduced quality. Incidences of abnormally developed larvae were more frequently observed among fish that hatched from the irradiated eggs. Observed changes suggest X‐rays applied for the genetic inactivation of rainbow trout eggs may lead to decrease of their developmental competence.
Author Marcin Polonis (FOG / IO / DMBE)
Marcin Polonis,,
- Department of Marine Biology and Ecology
, Krzysztof Jagiełło (FOG / IO / DMBE)
Krzysztof Jagiełło,,
- Department of Marine Biology and Ecology
, Stefan Dobosz
Stefan Dobosz,,
-
, Rafał Rożyński
Rafał Rożyński,,
-
, Paulina Kondraciuk (FOG / IO)
Paulina Kondraciuk,,
- Institute of Oceanography
, Artur Gurgul
Artur Gurgul,,
-
, Tomasz Szmatoła
Tomasz Szmatoła,,
-
, Konrad Ocalewicz (FOG / IO / DMBE)
Konrad Ocalewicz,,
- Department of Marine Biology and Ecology
Journal seriesReproduction in Domestic Animals, ISSN 0936-6768, (N/A 100 pkt)
Issue year2019
Vol54
No4
Pages712-718
Publication size in sheets0.5
Keywords in Englishandrogenesis, body malformations, egg quality, lipid droplets, reactive oxygen species
ASJC Classification1310 Endocrinology; 1103 Animal Science and Zoology; 1305 Biotechnology
DOIDOI:10.1111/rda.13413
URL https://onlinelibrary.wiley.com/doi/epdf/10.1111/rda.13413
Languageen angielski
Score (nominal)100
ScoreMinisterial score = 100.0, 30-09-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.934; WoS Impact Factor: 2017 = 1.422 (2) - 2017=1.599 (5)
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