Protein aggregation in Ehrlichia chaffeensis during infection of mammalian cells

Dorota Kuczyńska-Wiśnik , Chuanmin Cheng , Roman R. Ganta , Michal Zolkiewski

Abstract

Ehrlichia chaffeensis is an obligatory intracellular pathogen transmitted through infected ticks to humans and other vertebrates. We investigated the extent of protein aggregation in E. chaffeensis during infection of canine macrophage cell line, DH82. We discovered that the size of the aggregated fraction of E. chaffeensis proteins increased during the first 48 h post infection. We also incubated the infected cells with guanidinium chloride (GuHCl), a known inhibitor of the protein-disaggregating molecular chaperone ClpB. Up to 0.5 mM GuHCl had no impact on the host cells, whereas the viability of the pathogen was reduced by ∼60% in the presence of the inhibitor. Furthermore, we found that the size of the aggregated protein fraction in E. chaffeensis increased significantly in cultures supplemented with 0.5 mM GuHCl, which also resulted in the preferential accumulation of ClpB with the aggregated proteins. Altogether, our results suggest that an exposure of E. chaffeensis to the stressful environment of a host cell results in an increased aggregation of the pathogen's proteins, which is exacerbated upon inhibition of ClpB. Our studies establish a link between protein quality control and pathogen survival during infection of a host.
Author Dorota Kuczyńska-Wiśnik (FB / DB)
Dorota Kuczyńska-Wiśnik,,
- Department of Biochemistry
, Chuanmin Cheng
Chuanmin Cheng,,
-
, Roman R. Ganta
Roman R. Ganta,,
-
, Michal Zolkiewski
Michal Zolkiewski,,
-
Journal seriesFEMS Microbiology Letters, ISSN 0378-1097, (A 20 pkt)
Issue year2017
Vol364
No6
Pages1-6
Publication size in sheets0.5
Keywords in EnglishEhrlichia chaffeensis, host-pathogen interaction, protein folding, protein aggregation, molecular chaperone, ClpB
DOIDOI:10.1093/femsle/fnx059
URL https://doi.org/10.1093/femsle/fnx059
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 20.0, 26-02-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 26-02-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.765 (2) - 2016=2.102 (5)
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