The metabolic shift in highly and weakly virulent Dickeya solani strains is more affected by temperature than by mutations in genes encoding global virulence regulators

Marta Potrykus , Francesca Decorosi , Izabela Perkowska , Carlo Viti , Alessio Mengoni , Nicole Hugouvieux-Cotte-Pattat , Ewa Łojkowska

Abstract

Global warming may shortly increase the risk of disease development on plants. Significant differences in the metabolic activity screened with Phenotype Microarray at 22°C and 28°C were observed between D. solani strains with high and low virulence level. Highly virulent D. solani was characterized by a higher number of metabolized compounds and a faster metabolism and was more tolerant to non-favorable pH and osmolarity. Metabolic phenotyping showed for the first time that the mutation in pecT gene, which encodes a global repressor of virulence, affects several pathways of the basic cell metabolism. PecT mutants had a higher maceration capacity of potato tissue and showed a higher pectinolytic activity than the wild-type strains. On the contrary, mutation in expI gene, which encoded the signaling molecules synthase crucial for quorum sensing, had an insignificant effect on the cell metabolism, although it slightly reduced the potato tissue maceration. The ability to utilize most of the tested compounds was higher at 28°C, while the survival at non-favorable pH and osmolarity was higher at 22°C. These results proved that the temperature of incubation had the most significant impact on the D. solani metabolic profiles.
Author Marta Potrykus (IFB/IB/DBiotech) - [Medical Uniwersity of Gdańsk (GUMed)]
Marta Potrykus,,
- Department of Biotechnology
- Gdański Uniwersytet Medyczny
, Francesca Decorosi
Francesca Decorosi ,,
-
, Izabela Perkowska (IFB/IB/DBiotech)
Izabela Perkowska,,
- Department of Biotechnology
, Carlo Viti
Carlo Viti,,
-
, Alessio Mengoni
Alessio Mengoni,,
-
, Nicole Hugouvieux-Cotte-Pattat
Nicole Hugouvieux-Cotte-Pattat,,
-
, Ewa Łojkowska (IFB/IB/DBiotech)
Ewa Łojkowska,,
- Department of Biotechnology
Journal seriesFEMS Microbiology Ecology, ISSN 0168-6496, e-ISSN 1574-6941, (N/A 100 pkt)
Issue year2020
Vol96
No3
Pages1-13
Publication size in sheets0.60
Article numberfiaa023
Keywords in EnglishPhenotype MicroarrayTM, pecT, expI, quorum sensing
ASJC Classification2402 Applied Microbiology and Biotechnology; 2404 Microbiology; 2303 Ecology
DOIDOI:10.1093/femsec/fiaa023
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 12-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.049; WoS Impact Factor: 2018 = 4.098 (2) - 2018=4.516 (5)
Citation count*
Cite
busy
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?