Synergic combinations of antimicrobial peptides (AMPs) against biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on polystyrene and medical devices

Eleonora Ciandrini , Gianluca Morroni , Oscar Cirioni , Wojciech Kamysz , Elżbieta Kamysz , Lucia Brescini , Wally Baffone , Raffaella Campana

Abstract

Objectives: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistantStaphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appears a promising solution. The aim of the present study was to investigate the potential activity of Temporin A, Citropin 1.1, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2 in synergic activity against MRSA biofilms developed on polystyrene surface (PSS) and central venous catheter (CVC). Methods: The research was subdivided into distinct phases to assess the ability of AMPs to inhibit biofilm formation, to identify a possible synergy between AMPs, and to eradicate preformed biofilms on PSS and CVC using AMPs alone or in combination. Results The activity of the AMPs was particularly evident in the inhibition of biofilm formation on PSS and on CVC, while the eradication of preformed biofilms was more difficult and was reached only after 24 h of contact. The synergic activity of AMPs combinations, selected by their FICI, has led to an improvement in the performance of all the molecules in the removal of different biofilms. Conclusions: Overall, AMPs could represent the next generation of antimicrobial agents for a prophylactic or therapeutic tool to control biofilm of antibiotic-resistant and/or biofilm-associated infections on different medical devices.
Publication typeIn press (online first, early view)
Author Eleonora Ciandrini
Eleonora Ciandrini,,
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, Gianluca Morroni
Gianluca Morroni,,
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, Oscar Cirioni
Oscar Cirioni,,
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, Wojciech Kamysz
Wojciech Kamysz,,
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, Elżbieta Kamysz (FCh/DMBt/LChBM)
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Lucia Brescini
Lucia Brescini,,
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, Wally Baffone
Wally Baffone,,
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, Raffaella Campana
Raffaella Campana,,
-
Journal seriesJournal of Global Antimicrobial Resistance, ISSN 2213-7165, e-ISSN 2213-7173, (N/A 40 pkt)
Issue year2019
Noonline first
Pages1-28
Publication size in sheets1.35
Keywords in Englishsynergistic activity, antimicrobial peptides, Staphylococcus aureus (MRSA), biofilmsmedical devices
ASJC Classification2723 Immunology and Allergy; 2726 Microbiology (medical); 2403 Immunology; 2404 Microbiology
DOIDOI:10.1016/j.jgar.2019.10.022
URL https://doi.org/10.1016/j.jgar.2019.10.022
Languageen angielski
File
Ciandrini_Eleonora_Synergic_combinations_2019.pdf 2,66 MB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 27-07-2020, ArticleFromJournal
Publication indicators WoS Citations = 1.000; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.890; WoS Impact Factor: 2018 = 2.469 (2) - 2018=2.352 (5)
Citation count*2 (2020-08-10)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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