Synergic combinations of antimicrobial peptides (AMPs) against biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on polystyrene and medical devices
Eleonora Ciandrini , Gianluca Morroni , Oscar Cirioni , Wojciech Kamysz , Elżbieta Kamysz , Lucia Brescini , Wally Baffone , Raffaella Campana
AbstractObjectives: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistantStaphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appears a promising solution. The aim of the present study was to investigate the potential activity of Temporin A, Citropin 1.1, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2 in synergic activity against MRSA biofilms developed on polystyrene surface (PSS) and central venous catheter (CVC). Methods: The research was subdivided into distinct phases to assess the ability of AMPs to inhibit biofilm formation, to identify a possible synergy between AMPs, and to eradicate preformed biofilms on PSS and CVC using AMPs alone or in combination. Results The activity of the AMPs was particularly evident in the inhibition of biofilm formation on PSS and on CVC, while the eradication of preformed biofilms was more difficult and was reached only after 24 h of contact. The synergic activity of AMPs combinations, selected by their FICI, has led to an improvement in the performance of all the molecules in the removal of different biofilms. Conclusions: Overall, AMPs could represent the next generation of antimicrobial agents for a prophylactic or therapeutic tool to control biofilm of antibiotic-resistant and/or biofilm-associated infections on different medical devices.
|Journal series||Journal of Global Antimicrobial Resistance, ISSN 2213-7165, e-ISSN 2213-7173, (N/A 40 pkt)|
|Publication size in sheets||1.35|
|Keywords in English||synergistic activity, antimicrobial peptides, Staphylococcus aureus (MRSA), biofilmsmedical devices|
|ASJC Classification||; ; ;|
|Score||= 40.0, 27-07-2020, ArticleFromJournal|
|Publication indicators||= 1.000; : 2018 = 0.890; : 2018 = 2.469 (2) - 2018=2.352 (5)|
|Citation count*||2 (2020-08-10)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.