A short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotency

Diana Coronado , Murielle Godet , Pierre-Yves Bourillot , Yann Tapponnier , Agnieszka Bernat , Maxime Petit , Marielle Afanassieff , Suzy Markossian , Anna Malashicheva , Roberto Iacone , Konstantinos Anastassiadis , Pierre Savatier

Abstract

A short G1 phase is a characteristic feature of mouse embryonic stem cells (ESCs). To determine if there is a causal relationship between G1 phase restriction and pluripotency, we made use of the Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) reporter system to FACS-sort ESCs in the different cell cycle phases. Hence, the G1 phase cells appeared to be more susceptible to differentiation, particularly when ESCs self-renewed in the naïve state of pluripotency. Transitions from ground to naïve, then from naïve to primed states of pluripotency were associated with increased durations of the G1 phase, and cyclin E-mediated alteration of the G1/S transition altered the balance between self-renewal and differentiation. LIF withdrawal resulted in a lengthening of the G1 phase in naïve ESCs, which occurred prior to the appearance of early lineage-specific markers, and could be reversed upon LIF supplementation. We concluded that the short G1 phase observed in murine ESCs was a determinant of naïve pluripotency and was partially under the control of LIF signaling.
Author Diana Coronado
Diana Coronado,,
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, Murielle Godet
Murielle Godet,,
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, Pierre-Yves Bourillot
Pierre-Yves Bourillot,,
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, Yann Tapponnier
Yann Tapponnier,,
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, Agnieszka Bernat (IFB / IB / DBiotech)
Agnieszka Bernat,,
- Department of Biotechnology
, Maxime Petit
Maxime Petit,,
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, Marielle Afanassieff
Marielle Afanassieff,,
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, Suzy Markossian
Suzy Markossian,,
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, Anna Malashicheva
Anna Malashicheva,,
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, Roberto Iacone
Roberto Iacone,,
-
et al.`
Journal seriesStem Cell Research, ISSN 1873-5061, (A 30 pkt)
Issue year2013
Vol10
No1
Pages118-131
Publication size in sheets0.65
DOIDOI:10.1016/j.scr.2012.10.004
URL http://dx.doi.org/10.1016/j.scr.2012.10.004
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Not used for evaluationyes
Score (nominal)0
ScoreMinisterial score = 35.0, 26-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 26-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2013 = 3.912 (2) - 2013=4.382 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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