Growth of bacterial phytopathogens in animal manures

Wojciech Śledź , Sabina Żołędowska , Agata Motyka , Leszek Kadziński , Bogdan Banecki


Animal manures are routinely applied to agricultural lands to improve crop yield, but the possibility to spread bacterial phytopathogens through field fertilization has not been considered yet. We monitored 49 cattle, horse, swine, sheep or chicken manure samples collected in 14 Polish voivodeships for the most important plant pathogenic bacteria - Ralstonia solanacearum (Rsol), Xanthomonas campestris pv. campestris (Xcc), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pectobacterium atrosepticum (Pba), Erwinia amylovora (Eam), Clavibacter michiganensis subsp. sepedonicus (Cms) and Dickeya sp. (Dsp). All of the tested animal fertilizers were free of these pathogens. Subsequently, the growth dynamics of Pba, Pcc, Rsol, and Xcc in cattle, horse, swine, sheep and chicken manures sterilized either by autoclaving or filtration was evaluated. The investigated phytopathogens did not exhibit any growth in the poultry manure. However, the manure filtrates originating from other animals were suitable for microbial growth, which resulted in the optical density change of 0.03-0.22 reached within 26 h (48 h Rsol, 120 h Xcc), depending on bacterial species and the manure source. Pcc and Pba multiplied most efficiently in the cattle manure filtrate. These bacteria grew faster than Rsol and Xcc in all the tested manure samples, both the filtrates and the autoclaved semi-solid ones. Though the growth dynamics of investigated strains in different animal fertilizers was unequal, all of the tested bacterial plant pathogens were proven to use cattle, horse, swine and sheep manures as the sources of nutrients. These findings may contribute to further research on the alternative routes of spread of bacterial phytopathogens, especially because of the fact that the control of pectionolytic bacteria is only based on preventive methods.
Author Wojciech Śledź (IFB / IB / DBiotech)
Wojciech Śledź,,
- Department of Biotechnology
, Sabina Żołędowska (IFB)
Sabina Żołędowska,,
- Intercollegiate Faculty of Biotechnology UG
, Agata Motyka (IFB)
Agata Motyka,,
- Intercollegiate Faculty of Biotechnology UG
, Leszek Kadziński (IFB / M020 / DMCB)
Leszek Kadziński,,
- Department of Molecular and Cellular Biology
, Bogdan Banecki (IFB / M020 / DMCB)
Bogdan Banecki,,
- Department of Molecular and Cellular Biology
Other language title versions
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, (A 15 pkt)
Issue year2017
Publication size in sheets0.5
Keywords in Englishanimal manure, plant protection, Ralstonia solanacearum, Xanthomonas campestris, Erwinia amylovora, Dickeya solani, Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum
Languageen angielski
LicenseJournal (articles only); published final; Other open licence; with publication
Score (nominal)15
ScoreMinisterial score = 15.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 1.159 (2) - 2016=1.491 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.