First report of saxitoxins and anatoxin-a production by cyanobacteria from Lithuanian lakes

Jūratė Karosienė , Ksenija Savadova-Ratkus , Anna Toruńska-Sitarz , Judita Koreivienė , Jūratė Kasperovičienė , Irma Vitonytė , Agata Błaszczyk , Hanna Mazur-Marzec

Abstract

This study describes the first report on saxitoxins (STXs) and anatoxin-a (ANTX-a) production by cyanobacteria in Lithuanian lakes. Seasonal fluctuation patterns for neurotoxins were determined in samples collected from eutrophic lakes during July - September. Strains of Aphanizomenon gracile, A. flos-aquae, Cuspidothrix issatschenkoi, Sphaerospermopsis aphanizomenoides and Anabaenopsis cf. elenkinii were also isolated from the lakes and screened for the occurrence of neurotoxic STXs, ANTX-a and for the cytotoxin cylindrospermopsin (CYN). The highest concentration of STX (up to 1.06 µg l-1 in Lake Jieznas) was detected during July - August and coincided with a predominance of A. gracile in the phytoplankton. Of the isolated strains, only A. gracile was confirmed to be a producer of STXs by chemical (LC-MS/MS) and genetic (sxtA) methods. Sequencing of the phycocyanin intergenic spacer and flanking regions (cpcBA) revealed that A. gracile formed a genetically homogenous group. Only trace amounts of ANTX-a were detected in field samples (up to 0.31 µg l-1 in Lake Širvys). CYN was detected in neither the bloom samples nor in the isolated strains.
Publication typeIn press (online first, early view)
Author Jūratė Karosienė
Jūratė Karosienė,,
-
, Ksenija Savadova-Ratkus
Ksenija Savadova-Ratkus,,
-
, Anna Toruńska-Sitarz (FOG / IO / DMarB)
Anna Toruńska-Sitarz,,
- Division of Marine Biotechnology
, Judita Koreivienė
Judita Koreivienė,,
-
, Jūratė Kasperovičienė
Jūratė Kasperovičienė,,
-
, Irma Vitonytė
Irma Vitonytė,,
-
, Agata Błaszczyk (FOG / IO / DMarB)
Agata Błaszczyk,,
- Division of Marine Biotechnology
, Hanna Mazur-Marzec (FOG / IO / DMarB)
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
Journal seriesEuropean Journal of Phycology, ISSN 0967-0262, e-ISSN 1469-4433, (N/A 70 pkt)
Issue year2020
Noonline first
Pages1-1
Publication size in sheets0.55
Keywords in EnglishAphanizomenon gracile, cyanobacteria, neurotoxins, PC-IGS, sxtA gene
ASJC Classification1104 Aquatic Science; 1110 Plant Science
DOIDOI:10.1080/09670262.2020.1734667
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 07-05-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.042; WoS Impact Factor: 2018 = 2.526 (2) - 2018=2.621 (5)
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