First report of saxitoxins and anatoxin-a production by cyanobacteria from Lithuanian lakes
Jūratė Karosienė , Ksenija Savadova-Ratkus , Anna Toruńska-Sitarz , Judita Koreivienė , Jūratė Kasperovičienė , Irma Vitonytė , Agata Błaszczyk , Hanna Mazur-Marzec
AbstractThis study describes the first report on saxitoxins (STXs) and anatoxin-a (ANTX-a) production by cyanobacteria in Lithuanian lakes. Seasonal fluctuation patterns for neurotoxins were determined in samples collected from eutrophic lakes during July - September. Strains of Aphanizomenon gracile, A. flos-aquae, Cuspidothrix issatschenkoi, Sphaerospermopsis aphanizomenoides and Anabaenopsis cf. elenkinii were also isolated from the lakes and screened for the occurrence of neurotoxic STXs, ANTX-a and for the cytotoxin cylindrospermopsin (CYN). The highest concentration of STX (up to 1.06 µg l-1 in Lake Jieznas) was detected during July - August and coincided with a predominance of A. gracile in the phytoplankton. Of the isolated strains, only A. gracile was confirmed to be a producer of STXs by chemical (LC-MS/MS) and genetic (sxtA) methods. Sequencing of the phycocyanin intergenic spacer and flanking regions (cpcBA) revealed that A. gracile formed a genetically homogenous group. Only trace amounts of ANTX-a were detected in field samples (up to 0.31 µg l-1 in Lake Širvys). CYN was detected in neither the bloom samples nor in the isolated strains.
|Journal series||European Journal of Phycology, ISSN 0967-0262, e-ISSN 1469-4433, (N/A 70 pkt)|
|Publication size in sheets||0.55|
|Keywords in English||Aphanizomenon gracile, cyanobacteria, neurotoxins, PC-IGS, sxtA gene|
|Score||= 70.0, 07-05-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 1.042; : 2018 = 2.526 (2) - 2018=2.621 (5)|
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