Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices

Gabor Jarvas , Andras Guttman , Natalia Miękus , Tomasz Bączek , Sunkyung Jeong , Doo Soo Chung , Vladimir Pätoprstý , Marián Masár , Milan Hutta , Vladimira Datinská , Frantisek Foret

Abstract

By coupling a sample pretreatment technique of sample clean up and enrichment power with capillary electrophoresis (CE) of high-performance separation, the task of analyzing trace analytes in a complex matrix such as a biological sample can be carried out successfully with ease. This review aims for providing an overview of strategies to couple sample pretreatment techniques with capillary and related microscale (e.g., microchip) electrophoresis, practically adoptable in an automatic manner, without requiring serious modification of existing instruments to install sophisticated interfaces. In-line sample pretreatment techniques based on liquid phase microextraction performed before sample injection and on-line sample preconcentration techniques performed during or after sample injection are discussed with emphasis on the applicability to samples of high conductivity, commonly encountered for biological samples. An overview of the recent developments in microfluidic immobilized enzymatic microreactors which fit excellently to microchip CE is also given.
Author Gabor Jarvas
Gabor Jarvas,,
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, Andras Guttman
Andras Guttman,,
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, Natalia Miękus (FB / DAHP)
Natalia Miękus,,
- Department of Animal and Human Physiology
, Tomasz Bączek
Tomasz Bączek,,
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, Sunkyung Jeong
Sunkyung Jeong,,
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, Doo Soo Chung
Doo Soo Chung,,
-
, Vladimir Pätoprstý
Vladimir Pätoprstý,,
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, Marián Masár
Marián Masár,,
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, Milan Hutta
Milan Hutta,,
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, Vladimira Datinská
Vladimira Datinská,,
-
et al.`
Journal seriesTrac-Trends in Analytical Chemistry, [TrAC - Trends in Analytical Chemistry], ISSN 0165-9936, e-ISSN 1879-3142, (N/A 140 pkt)
Issue year2020
Vol122
Pages1-9
Publication size in sheets0.5
Article number115702
Keywords in EnglishAnalyte preconcentration, capillary electrophoresis, liquid phase separation, microreactors, sample pretreatment
ASJC Classification1602 Analytical Chemistry; 1607 Spectroscopy
DOIDOI:10.1016/j.trac.2019.115702
URL https://doi.org/10.1016/j.trac.2019.115702
Languageen angielski
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 2.004; WoS Impact Factor: 2018 = 8.428 (2) - 2018=8.742 (5)
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