A recombinant turkey herpesvirus expressing F and HN genes of Avian avulavirus-1 (AAvV-1) genotype VI confers cross-protection against challenge with virulent AAvV-1 genotypes IV and VII in chickens

Krzysztof Śmietanka , Jolanta Tyborowska , Monika Olszewska-Tomczyk , Katarzyna Domańska-Blicharz , Zenon Minta , Łukasz Rąbalski , Anna Czarnota , Krzysztof Kucharczyk , Bogusław Szewczyk

Abstract

Newcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeon variant” of AAvV-1). This recombinant viral vaccine candidate was used to subcutaneously immunize one group of specific pathogen-free (SPF) chickens and two groups of broiler chickens (20 one-day-old birds/group). Humoral immune response was evaluated by hemagglutination-inhibition test and enzyme-linked immunosorbent assay (ELISA). The efficacy of the immunization was assessed in two separate challenge studies performed at 6 weeks of age with the use of virulent AAvV-1 strains representing heterologous genotypes IV and VII. The developed vaccine candidate elicited complete protection in SPF chickens since none of the birds became sick or died during the 2-week observation period. In the broiler groups, 90% and 100% clinical protection were achieved after challenges with AAvV-1 of IV and VII genotypes, respectively. We found no obvious relationship between antibody levels and protection assessed in broilers in the challenge study. The developed recombinant HVT-NDV-F-HN construct containing genes from a genotype VI AAvV-1 offers promising results as a potential vaccine candidate against ND in chickens.
Author Krzysztof Śmietanka
Krzysztof Śmietanka,,
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, Jolanta Tyborowska (IFB / IB / LRV)
Jolanta Tyborowska,,
- Laboratory of Recombinant Vaccines
, Monika Olszewska-Tomczyk
Monika Olszewska-Tomczyk,,
-
, Katarzyna Domańska-Blicharz
Katarzyna Domańska-Blicharz,,
-
, Zenon Minta
Zenon Minta,,
-
, Łukasz Rąbalski (IFB / IB / LRV)
Łukasz Rąbalski,,
- Laboratory of Recombinant Vaccines
, Anna Czarnota (IFB / IB / LVMB)
Anna Czarnota,,
- Laboratory of Virus Molecular Biology
, Krzysztof Kucharczyk
Krzysztof Kucharczyk,,
-
, Bogusław Szewczyk (IFB / IB / LRV)
Bogusław Szewczyk,,
- Laboratory of Recombinant Vaccines
Journal seriesViruses-Basel, [Viruses], ISSN 1999-4915, (N/A 100 pkt)
Issue year2019
Vol11
No9
Pages1-13
Publication size in sheets0.6
Article number784
Keywords in EnglishNewcastle disease, avian avulavirus 1, recombinant vector vaccine
ASJC Classification2725 Infectious Diseases; 2406 Virology
DOIDOI:10.3390/v11090784
URL https://www.mdpi.com/1999-4915/11/9/784/pdf
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 05-03-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.018; WoS Impact Factor: 2018 = 3.811 (2) - 2018=3.916 (5)
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