A recombinant turkey herpesvirus expressing F and HN genes of Avian avulavirus-1 (AAvV-1) genotype VI confers cross-protection against challenge with virulent AAvV-1 genotypes IV and VII in chickens
Krzysztof Śmietanka , Jolanta Tyborowska , Monika Olszewska-Tomczyk , Katarzyna Domańska-Blicharz , Zenon Minta , Łukasz Rąbalski , Anna Czarnota , Krzysztof Kucharczyk , Bogusław Szewczyk
AbstractNewcastle disease (ND) is responsible for significant economic losses in the poultry industry. The disease is caused by virulent strains of Avian avulavirus 1 (AAvV-1), a species within the family Paramyxoviridae. We developed a recombinant construct based on the herpesvirus of turkeys (HVT) as a vector expressing two genes: F and HN (HVT-NDV-F-HN) derived from the AAvV-1 genotype VI (“pigeon variant” of AAvV-1). This recombinant viral vaccine candidate was used to subcutaneously immunize one group of specific pathogen-free (SPF) chickens and two groups of broiler chickens (20 one-day-old birds/group). Humoral immune response was evaluated by hemagglutination-inhibition test and enzyme-linked immunosorbent assay (ELISA). The efficacy of the immunization was assessed in two separate challenge studies performed at 6 weeks of age with the use of virulent AAvV-1 strains representing heterologous genotypes IV and VII. The developed vaccine candidate elicited complete protection in SPF chickens since none of the birds became sick or died during the 2-week observation period. In the broiler groups, 90% and 100% clinical protection were achieved after challenges with AAvV-1 of IV and VII genotypes, respectively. We found no obvious relationship between antibody levels and protection assessed in broilers in the challenge study. The developed recombinant HVT-NDV-F-HN construct containing genes from a genotype VI AAvV-1 offers promising results as a potential vaccine candidate against ND in chickens.
|Journal series||Viruses-Basel, [Viruses], ISSN 1999-4915, (N/A 100 pkt)|
|Publication size in sheets||0.6|
|Keywords in English||Newcastle disease, avian avulavirus 1, recombinant vector vaccine|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 05-03-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2016 = 1.018; : 2018 = 3.811 (2) - 2018=3.916 (5)|
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