Sensitive detection of single-cell secreted H2O2 by integrating a microfluidic droplet sensor and Au nanoclusters
Rui Shen , Peipei Liu , Yiqiu Zhang , Zhao Yu , Xuyue Chen , Lu Zhou , Baoqing Nie , Anna Żaczek , Jian Chen , Jian Liu
AbstractAs an important signaling molecule, hydrogen peroxide (H2O2) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H2O2 secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H2O2 can initiate dramatic fluorescence changes of horseradish peroxidase−Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200−400 attomole H2O2 directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H2O2 secretion at the single-cell level.
|Journal series||Analytical Chemistry, ISSN 0003-2700, (A 45 pkt)|
|Publication size in sheets||0.5|
|Score|| = 45.0, ArticleFromJournal|
= 45.0, ArticleFromJournal
|Publication indicators||: 2017 = 6.042 (2) - 2017=6.035 (5)|
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