Sensitive detection of single-cell secreted H2O2 by integrating a microfluidic droplet sensor and Au nanoclusters

Rui Shen , Peipei Liu , Yiqiu Zhang , Zhao Yu , Xuyue Chen , Lu Zhou , Baoqing Nie , Anna Żaczek , Jian Chen , Jian Liu

Abstract

As an important signaling molecule, hydrogen peroxide (H2O2) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H2O2 secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H2O2 can initiate dramatic fluorescence changes of horseradish peroxidase−Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200−400 attomole H2O2 directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H2O2 secretion at the single-cell level.
Author Rui Shen
Rui Shen,,
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, Peipei Liu
Peipei Liu,,
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, Yiqiu Zhang
Yiqiu Zhang,,
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, Zhao Yu
Zhao Yu,,
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, Xuyue Chen
Xuyue Chen,,
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, Lu Zhou
Lu Zhou,,
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, Baoqing Nie
Baoqing Nie,,
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, Anna Żaczek KBM
Anna Żaczek,,
- Department of Medical Biotechnology
, Jian Chen
Jian Chen,,
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, Jian Liu
Jian Liu,,
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Journal seriesAnalytical Chemistry, ISSN 0003-2700
Issue year2018
Vol90
No7
Pages4478-4484
Publication size in sheets0.5
DOIDOI:10.1021/acs.analchem.7b04798
URL https://pubs.acs.org/doi/10.1021/acs.analchem.7b04798
Languageen angielski
Score (nominal)45
ScoreMinisterial score = 45.0, 30-05-2018, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 30-05-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 6.32 (2) - 2016=6.016 (5)
Citation count*0
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