Sensitive detection of single-cell secreted H2O2 by integrating a microfluidic droplet sensor and Au nanoclusters

Rui Shen , Peipei Liu , Yiqiu Zhang , Zhao Yu , Xuyue Chen , Lu Zhou , Baoqing Nie , Anna Żaczek , Jian Chen , Jian Liu


As an important signaling molecule, hydrogen peroxide (H2O2) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H2O2 secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H2O2 can initiate dramatic fluorescence changes of horseradish peroxidase−Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200−400 attomole H2O2 directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H2O2 secretion at the single-cell level.
Author Rui Shen
Rui Shen,,
, Peipei Liu
Peipei Liu,,
, Yiqiu Zhang
Yiqiu Zhang,,
, Zhao Yu
Zhao Yu,,
, Xuyue Chen
Xuyue Chen,,
, Lu Zhou
Lu Zhou,,
, Baoqing Nie
Baoqing Nie,,
, Anna Żaczek (IFB / DMedB)
Anna Żaczek,,
- Department of Medical Biotechnology
, Jian Chen
Jian Chen,,
, Jian Liu
Jian Liu,,
Journal seriesAnalytical Chemistry, ISSN 0003-2700, (A 45 pkt)
Issue year2018
Publication size in sheets0.5
ASJC Classification1602 Analytical Chemistry
Languageen angielski
Score (nominal)45
Score sourcejournalList
ScoreMinisterial score = 45.0, 28-01-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.486; WoS Impact Factor: 2018 = 6.35 (2) - 2018=6.337 (5)
Citation count*
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?