Copper(II) coordination properties of GxG peptides: Key role of side chains of central residues on coordination of formed systems; combined potentiometric and ITC studies

Joanna Makowska , Dariusz Wyrzykowski , Bogusław Pilarski , Damian Neubauer , Elżbieta Kamysz , Aleksandra Tesmar , Lech Chmurzyński

Abstract

Isothermal titration calorimetry (ITC) and potentiometric titration (PT) methods were used to study the interactions of copper(II) ions with GAG, GDG, GKG, and GHG peptides. The calorimetric measurements were run in a MES buffer with the pH value of 6.0 at 298.15 K. Based on the results of PT data supported by theoretical calculations, the dissociation constants were calculated for the investigated peptides. The quantification of the proton competition with the metal for the peptide and incorporation it into the ITC data analysis enabled to obtain the pH-independent parameters, namely the binding constants (K) and the free energy of binding (DG). Furthermore, the relationship between the proposed coordination modes of the considered peptides and the thermodynamic parameters (K, ΔG and ΔH) has been discussed.
Author Joanna Makowska (FCh / DGICh / PBChN)
Joanna Makowska,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Dariusz Wyrzykowski (FCh / DGICh / PBChN)
Dariusz Wyrzykowski,,
- Pracownia Biologicznej Chemii Nieorganicznej
, Bogusław Pilarski
Bogusław Pilarski,,
-
, Damian Neubauer
Damian Neubauer,,
-
, Elżbieta Kamysz (FCh / DMBt / LChBM)
Elżbieta Kamysz,,
- Laboratory of Chemistry of Biological Macromolecules
, Aleksandra Tesmar (FCh / DGICh / LPCC)
Aleksandra Tesmar,,
- Laboratory of Physicochemistry of Coordination Complexes
, Lech Chmurzyński (FCh / DGICh / LPCC)
Lech Chmurzyński,,
- Laboratory of Physicochemistry of Coordination Complexes
Journal seriesJournal of Chemical Thermodynamics, ISSN 0021-9614, (A 30 pkt)
Issue year2019
Vol128
Pages336-343
Publication size in sheets0.5
DOIDOI:10.1016/j.jct.2018.08.040
URL https://doi.org/10.1016/j.jct.2018.08.040
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 30.0, 08-01-2019, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 08-01-2019, ArticleFromJournal
Publication indicators WoS Impact Factor: 2017 = 2.631 (2) - 2017=2.531 (5)
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