Limited microcystin, anatoxin and cylindrospermopsin production by cyanobacteria from microbial mats in cold deserts

Nataliia Khomutovska , Małgorzata Sandzewicz , Łukasz Łach , Małgorzata Suska-Malawska , Monika Chmielewska , Hanna Mazur-Marzec , Marta Cegłowska , Toirbek Niyatbekov , Susanna A. Wood , Jonathan Puddick , Jan Kwiatowski , Iwona Jasser

Abstract

Toxic metabolites are produced by many cyanobacterial species. There are limited data on toxigenic benthic, mat-forming cyanobacteria, and information on toxic cyanobacteria from Central Asia is even more scarce. In the present study, we examined cyanobacterial diversity and community structure, the presence of genes involved in toxin production and the occurrence of cyanotoxins in cyanobacterial mats from small water bodies in a cold high-mountain desert of Eastern Pamir. Diversity was explored using amplicon-based sequencing targeting the V3-V4 region of the 16S rRNA gene, toxin potential using PCR-based methods (mcy, nda, ana, sxt), and toxins by enzyme-linked immunosorbent assays (ELISAs) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Molecular identification of cyanobacteria showed a high similarity of abundant taxa to Nostoc PCC-73102, Nostoc PCC-7524, Nodularia PCC-935 and Leptolyngbya CYN68. The PCRs revealed the presence of mcyE and/or ndaF genes in 11 samples and mcyD in six. The partial sequences of the mcyE gene showed high sequence similarity to Nostoc, Planktothrix and uncultured cyanobacteria. LC-MS/MS analysis identified six microcystin congeners in two samples and unknown peptides in one. These results suggest that, in this extreme environment, cyanobacteria do not commonly produce microcystins, anatoxins and cylindrospermopsins, despite the high diversity and widespread occurrence of potentially toxic taxa.
Author Nataliia Khomutovska
Nataliia Khomutovska,,
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, Małgorzata Sandzewicz
Małgorzata Sandzewicz,,
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, Łukasz Łach
Łukasz Łach,,
-
, Małgorzata Suska-Malawska
Małgorzata Suska-Malawska,,
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, Monika Chmielewska
Monika Chmielewska,,
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, Hanna Mazur-Marzec (FOG/IO/DMarB)
Hanna Mazur-Marzec,,
- Division of Marine Biotechnology
, Marta Cegłowska
Marta Cegłowska,,
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, Toirbek Niyatbekov
Toirbek Niyatbekov,,
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, Susanna A. Wood
Susanna A. Wood,,
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, Jonathan Puddick
Jonathan Puddick,,
-
et al.`
Journal seriesToxins, ISSN 2072-6651, (N/A 100 pkt)
Issue year2020
Vol12
No4
Pages1-19
Publication size in sheets0.90
Article number244
Keywords in Englishcyanotoxins, microbial mats, V3-V4 hypervariable region, 16S rRNA gene, mcyA, mcyE, mcyD, ndaF, anaC, sxtA, potential toxin producers
ASJC Classification3005 Toxicology; 2307 Health, Toxicology and Mutagenesis
DOIDOI:10.3390/toxins12040244
URL https://doi.org/10.3390/toxins12040244
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 16-04-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.136; WoS Impact Factor: 2018 = 3.895 (2) - 2018=4.009 (5)
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