Data regarding a new, vector-enzymatic DNA fragment amplification-expression technology for the construction of artificial, concatemeric DNA, RNA and proteins, as well as biological effects of selected polypeptides obtained using this method
Piotr Skowron , Natalia Krawczun , Joanna Żebrowska , Daria Krefft , Olga Żołnierkiewicz , Marta Bielawa , Joanna Jeżewska-Frąckowiak , Łukasz Janus , Małgorzata Witkowska , Małgorzata Palczewska , Adriana Schumacher , Anna Wardowska , Milena Deptuła , Artur Czupryn , Piotr Mucha , Arkadiusz Piotrowski , Paweł Sachadyn , Sylwia Rodziewicz-Motowidło , Michał Pikuła , Agnieszka Żylicz-Stachula
AbstractApplications of bioactive peptides and polypeptides are emerging in areas such as drug development and drug delivery systems. These compounds are bioactive, biocompatible and represent a wide range of chemical properties, enabling further adjustments of obtained biomaterials. However, delivering large quantities of peptide derivatives is still challenging. Several methods have been developed for the production of concatemers – multiple copies of the desired protein segments. We have presented an efficient method for the production of peptides of desired length, expressed from concatemeric Open Reading Frame. The method employs specific amplification-expression DNA vectors. The main methodological approaches are described by Skowron et al., 2020 . As an illustration of the demonstrated method’s utility, an epitope from the S protein of Hepatitis B virus (HBV) was amplified. Additionally, peptides, showing potentially pro-regenerative properties, derived from the angiopoietin-related growth factor (AGF) were designed and amplified. Here we present a dataset including: (i) detailed protocols for the purification of HBV and AGF – derived polyepitopic protein concatemers, (ii) sequences of the designed primers, vectors and recombinant constructs (iii) data on cytotoxicity, immunogenicity and stability of AGF-derived polypeptides.
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.