Data regarding a new, vector-enzymatic DNA fragment amplification-expression technology for the construction of artificial, concatemeric DNA, RNA and proteins, as well as biological effects of selected polypeptides obtained using this method

Piotr Skowron , Natalia Krawczun , Joanna Żebrowska , Daria Krefft , Olga Żołnierkiewicz , Marta Bielawa , Joanna Jeżewska-Frąckowiak , Łukasz Janus , Małgorzata Witkowska , Małgorzata Palczewska , Adriana Schumacher , Anna Wardowska , Milena Deptuła , Artur Czupryn , Piotr Mucha , Arkadiusz Piotrowski , Paweł Sachadyn , Sylwia Rodziewicz-Motowidło , Michał Pikuła , Agnieszka Żylicz-Stachula


Applications of bioactive peptides and polypeptides are emerging in areas such as drug development and drug delivery systems. These compounds are bioactive, biocompatible and represent a wide range of chemical properties, enabling further adjustments of obtained biomaterials. However, delivering large quantities of peptide derivatives is still challenging. Several methods have been developed for the production of concatemers – multiple copies of the desired protein segments. We have presented an efficient method for the production of peptides of desired length, expressed from concatemeric Open Reading Frame. The method employs specific amplification-expression DNA vectors. The main methodological approaches are described by Skowron et al., 2020 [1]. As an illustration of the demonstrated method’s utility, an epitope from the S protein of Hepatitis B virus (HBV) was amplified. Additionally, peptides, showing potentially pro-regenerative properties, derived from the angiopoietin-related growth factor (AGF) were designed and amplified. Here we present a dataset including: (i) detailed protocols for the purification of HBV and AGF – derived polyepitopic protein concatemers, (ii) sequences of the designed primers, vectors and recombinant constructs (iii) data on cytotoxicity, immunogenicity and stability of AGF-derived polypeptides.
Author Piotr Skowron (FCh/DMBt/LGE)
Piotr Skowron,,
- Laboratory of Genetic Engineering
, Natalia Krawczun (FCh/DMBt)
Natalia Krawczun,,
- Department of Molecular Biotechnology
, Joanna Żebrowska (FCh/DMBt/LGE)
Joanna Żebrowska,,
- Laboratory of Genetic Engineering
, Daria Krefft (FCh/DMBt/LGE)
Daria Krefft,,
- Laboratory of Genetic Engineering
, Olga Żołnierkiewicz (FCh/DMBt/LGE)
Olga Żołnierkiewicz,,
- Laboratory of Genetic Engineering
, Marta Bielawa
Marta Bielawa,,
, Joanna Jeżewska-Frąckowiak (FCh/DMBt/LGE)
Joanna Jeżewska-Frąckowiak,,
- Laboratory of Genetic Engineering
, Łukasz Janus (FCh/DMBt/LGE)
Łukasz Janus,,
- Laboratory of Genetic Engineering
, Małgorzata Witkowska (FCh/DMBt/LGE)
Małgorzata Witkowska,,
- Laboratory of Genetic Engineering
, Małgorzata Palczewska (FCh/DMBt/LGE)
Małgorzata Palczewska,,
- Laboratory of Genetic Engineering
et al.`
Journal seriesData in Brief, ISSN 2352-3409, (N/A 40 pkt)
Issue year2020
Publication size in sheets0.75
Article number105069
Referenced article (e.g. original article) Skowron Piotr, Krawczun Natalia, Żebrowska Joanna [et al.]: A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications, in: Materials Science & Engineering C - Materials for Biological Applications, vol. 108, 2020, pp. 1-15, Article number:110426, DOI:10.1016/j.msec.2019.110426
Keywords in EnglishConcatemeric polypeptides, DNA amplification, DNA multimers, peptide-based biomaterials
ASJC Classification1000 Multidisciplinary
Languageen angielski
LicenseJournal (articles only); author's final; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); with publication
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 05-06-2020, ArticleFromJournal
Publication indicators WoS Citations = 0.000; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.429
Citation count*
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?