Application of SPME supported by ionic liquids for the determination of biogenic amines by MEKC in clinical practice

Natalia Kossakowska , Ilona Olędzka , Alicja Kowalik , Natalia Miękus , Piotr Kowalski , Alina Plenis , Ewa Bień , Aleksandra Kaczorowska , Małgorzata Anna Krawczyk , Elżbieta Adamkiewicz-Drożyńska , Tomasz Bączek


The analysis of biogenic amines (BAs) and their metabolites is helpful for the diagnosis of central nervous system disorders and other neuroendocrine and cancer disturbances. In the study, a developed micellar electrokinetic chromatography method, coupled with diode array detection (MEKC-DAD), was validated to monitor levels of adrenaline (A), noradrenaline (NA), dopamine (DA), L-Tryptophan (L-Tryp) and L-Tyrosine (L-Tyr) in real human urine samples. These neurotransmitters were isolated from urine samples using solid-phase microextraction (SPME) and methanol containing 1-ethyl-3-methylimidazolium tetrafluoroborate ionic liquid as the desorption phase. The method was linear for DA, A and L-Tyr in the range of 0.5–20 μg/mL and for NA and L-Tryp in the range of 0.25–20 μg/mL. The good linearity for BAs was confirmed by the correlation coefficient (R2) from 0.9989 for A to 0.9997 for NA and L-Tryp, respectively. The validation assays for accuracy, precision, limit of detection, limit of quantification, absolute recovery, and stability of the analytes were consistent with the requirements recommended by the FDA and ICH guidelines. Next, the validated SPME-MEKC method was successfully used for the quantification of A, NA, DA, L-Tryp and L-Tyr in real human urine samples collected from pediatric patients suffering from neuroblastoma, ganglioneuroblastoma, Wilms’ tumor, rhabdoid tumor and lipoblastomatosis, as well as from healthy volunteers. Finally, the levels of BAs in cancer patients were evaluated as to whether they can be used as biomarkers of various health disturbances.
Author Natalia Kossakowska
Natalia Kossakowska,,
, Ilona Olędzka
Ilona Olędzka,,
, Alicja Kowalik
Alicja Kowalik,,
, Natalia Miękus (FB / DAHP)
Natalia Miękus,,
- Department of Animal and Human Physiology
, Piotr Kowalski
Piotr Kowalski,,
, Alina Plenis
Alina Plenis,,
, Ewa Bień
Ewa Bień,,
, Aleksandra Kaczorowska
Aleksandra Kaczorowska,,
, Małgorzata Anna Krawczyk
Małgorzata Anna Krawczyk,,
, Elżbieta Adamkiewicz-Drożyńska
Elżbieta Adamkiewicz-Drożyńska,,
et al.`
Journal seriesJournal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, (N/A 100 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in EnglishIonic liquids (ILs), biogenic amines (BAs), micellar electrokinetic chromatography (MEKC), solid-phase microextraction (SPME), urine samples
ASJC Classification1308 Clinical Biochemistry; 1607 Spectroscopy; 3002 Drug Discovery; 3003 Pharmaceutical Science; 1602 Analytical Chemistry
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 28-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.144; WoS Impact Factor: 2018 = 2.983 (2) - 2018=3.054 (5)
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