Pectobacterium polonicum sp. nov. isolated from vegetable fields

Małgorzata Waleron , Agnieszka Misztak , Michał Waleron , Joanna Jońca , Magda A. Furmaniak , Krzysztof Waleron

Abstract

Gram-stain-negative, rod-shaped pectinolytic bacteria strains designated as DPMP315T, DPMP316, DPMP317 and DPMP318 isolated from groundwater sampled from a vegetable field in the North of Poland, were subjected to the polyphasic analyses. Multilocus sequence analyses based on five housekeeping genes (gyrA, recA, recN, rpoA and rpoS) revealed their distinctiveness from the other species of the genus, simultaneously indicating that the newly described species, Pectobacterium punjabense , as well as Pectobacterium parmentieri and P. wasabiae , to be the closest relatives. In silico DNA–DNA hybridization (<43.1 %) and average nucleotide identity (<92.5 %) values of strain DPMP315T with other type strains of species of the genus Pectobacterium supported the delineation of the novel strain as representing a novel species. The phenotypic comparisons, fatty acid methyl esters compositions, genetic rep PCR fingerprint and detailed whole-cell MALDI-TOF mass spectrometry proteomic profiles permitted the differentiation of Polish strains from the type strains of all other known species of the genus Pectobacterium . The results of polyphasic analyses performed for four Polish strains are the basis for the distinction of the novel species. Here, we propose to establish DPMP315T as a type strain (=PCM3006T=LMG 31077T) with the name Pectobacterium polonicum sp. nov.
Author Małgorzata Waleron (IFB / IB / DBiotech)
Małgorzata Waleron,,
- Department of Biotechnology
, Agnieszka Misztak (IFB)
Agnieszka Misztak,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Waleron (IFB)
Michał Waleron,,
- Intercollegiate Faculty of Biotechnology UG
, Joanna Jońca
Joanna Jońca,,
-
, Magda A. Furmaniak
Magda A. Furmaniak,,
-
, Krzysztof Waleron
Krzysztof Waleron,,
-
Journal seriesInternational Journal of Systematic and Evolutionary Microbiology, ISSN 1466-5026, (A 25 pkt)
Issue year2019
Vol69
No6
Pages1751-1759
Publication size in sheets0.5
Keywords in Englishgroundwater, Pectobacterium parmentieri, Pectobacterium punjabense, Pectobacterium wasabiae
ASJC Classification2700 General Medicine; 1105 Ecology, Evolution, Behavior and Systematics; 2404 Microbiology
DOIDOI:10.1099/ijsem.0.003387
Languageen angielski
Score (nominal)25
ScoreMinisterial score = 25.0, 24-07-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.171; WoS Impact Factor: 2017 = 1.932 (2) - 2017=2.385 (5)
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