Development and validation of a high-performance liquid chromatographic method with a fluorescence detector for the analysis of epirubicin in human urine and plasma, and its application in drug monitoring
Natalia Treder , Olga Maliszewska , Ilona Olędzka , Piotr Kowalski , Natalia Miękus , Tomasz Bączek , Ewa Bień , Małgorzata Anna Krawczyk , Elżbieta Adamkiewicz-Drożynska , Alina Plenis
AbstractThe aim of the work was to develop a simple, sensitive and accurate liquid chromatography with fluorescence detection (LC-FL) method for the determination of epirubicin in human urine and plasma. Solid phase extraction with HLB cartridges and mixture of dichloromethane:2-propanol:methanol (2:1:1, v/v/v) as the eluent, was used to prepare the samples. The chromatographic analysis was carried out on a Synergi Hydro-RP column with a mobile phase consisting of 40 mM phosphate buffer (pH 4.1) and acetonitrile (69:31, v/v). Epirubicin was monitored at 497 nm and 557 nm for excitation and emission wavelengths, respectively. Validation data confirmed that the limit of detection and limit of quantification was 0.25 ng/mL and 0.5 ng/mL in both matrices. Next, the optimized LC-FL method was applied to determine the level of epirubicin in real samples taken from a 19-year-old patient with metastatic alveolar rhabdomyosarcoma (RMA) to create a drug profile. Plasma and urine samples were collected for 24 h after the end of a 6-hour infusion of epirubicin. The obtained results confirmed that the optimized and validated LC-FL method can be successfully used in drug monitoring therapy, pharmacokinetic and clinical studies. Moreover, the current work is also drawing attention to the relatively high level of epirubicin in the patient urine, which requires compliance with the safety rules in contact with this biological fluid by both medical staff and others, e.g. family members.
|Journal series||Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, [Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences], ISSN 1570-0232, e-ISSN 1873-376X, (N/A 70 pkt)|
|Publication size in sheets||0.50|
|Keywords in English||Anthracyclines, epirubicin, liquid chromatography with fluorescence detection (LC-FL), human samples, solid-phase extraction|
|ASJC Classification||; ; ; ;|
|Score||= 70.0, 21-02-2020, ArticleFromJournal|
|Publication indicators||= 0.000; : 2018 = 1.028; : 2018 = 2.813 (2) - 2018=2.751 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.