Isolation and characterization of phages infecting Bacillus subtilis

Anna Krasowska , Anna Biegalska , Daria Augustyniak , Marcin Łoś , Malwina Richert , Marcin Łukaszewicz

Abstract

Bacteriophages have been suggested as an alternative approach to reduce the amount of pathogens in various applications. Bacteriophages of various specificity and virulence were isolated as a means of controlling food-borne pathogens. We studied the interaction of bacteriophages with Bacillus species, which are very often persistent in industrial applications such as food production due to their antibiotic resistance and spore formation. A comparative study using electron microscopy, PFGE, and SDS-PAGE as well as determination of host range, pH and temperature resistance, adsorption rate, latent time, and phage burst size was performed on three phages of the Myoviridae family and one phage of the Siphoviridae family which infected Bacillus subtilis strains. The phages are morphologically different and characterized by icosahedral heads and contractile (SIOΦ, SUBω, and SPOσ phages) or noncontractile (ARπ phage) tails. The genomes of SIOΦ and SUBω are composed of 154 kb. The capsid of SIOΦ is composed of four proteins. Bacteriophages SPOσ and ARπ have genome sizes of 25 kbp and 40 kbp, respectively. Both phages as well as SUBω phage have 14 proteins in their capsids. Phages SIOΦ and SPOσ are resistant to high temperatures and to the acid (4.0) and alkaline (9.0 and 10.0) pH.
Author Anna Krasowska
Anna Krasowska,,
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, Anna Biegalska
Anna Biegalska,,
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, Daria Augustyniak
Daria Augustyniak,,
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, Marcin Łoś (FB / DMGB)
Marcin Łoś,,
- Department of Molecular Genetics of Bacteria
, Malwina Richert (FB / LEM)
Malwina Richert,,
- Laboratory of Electron Microscopy
, Marcin Łukaszewicz
Marcin Łukaszewicz,,
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Journal seriesBioMed Research International, [Journal of Biomedicine and Biotechnology], ISSN 2314-6133, e-ISSN 2314-6141, (A 25 pkt)
Issue year2015
Vol2015
Pages1-10
Publication size in sheets0.3
Keywords in Englishbacteriophage, Bacillus subtilis, morphology
DOIDOI:10.1155/2015/179597
URL http://dx.doi.org/10.1155/2015/179597
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)25
ScoreMinisterial score = 20.0, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, ArticleFromJournal
Publication indicators WoS Impact Factor: 2015 = 2.134 (2) - 2015=2.149 (5); WoS Citations = 9
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