Isolation and characterization of phages infecting Bacillus subtilis
Anna Krasowska , Anna Biegalska , Daria Augustyniak , Marcin Łoś , Malwina Richert , Marcin Łukaszewicz
AbstractBacteriophages have been suggested as an alternative approach to reduce the amount of pathogens in various applications. Bacteriophages of various specificity and virulence were isolated as a means of controlling food-borne pathogens. We studied the interaction of bacteriophages with Bacillus species, which are very often persistent in industrial applications such as food production due to their antibiotic resistance and spore formation. A comparative study using electron microscopy, PFGE, and SDS-PAGE as well as determination of host range, pH and temperature resistance, adsorption rate, latent time, and phage burst size was performed on three phages of the Myoviridae family and one phage of the Siphoviridae family which infected Bacillus subtilis strains. The phages are morphologically different and characterized by icosahedral heads and contractile (SIOΦ, SUBω, and SPOσ phages) or noncontractile (ARπ phage) tails. The genomes of SIOΦ and SUBω are composed of 154 kb. The capsid of SIOΦ is composed of four proteins. Bacteriophages SPOσ and ARπ have genome sizes of 25 kbp and 40 kbp, respectively. Both phages as well as SUBω phage have 14 proteins in their capsids. Phages SIOΦ and SPOσ are resistant to high temperatures and to the acid (4.0) and alkaline (9.0 and 10.0) pH.
|Journal series||BioMed Research International, [Journal of Biomedicine and Biotechnology], ISSN 2314-6133, e-ISSN 2314-6141, (A 25 pkt)|
|Publication size in sheets||0.3|
|Keywords in English||bacteriophage, Bacillus subtilis, morphology|
|License||Journal (articles only); published final; ; with publication|
|Score|| = 20.0, ArticleFromJournal|
= 30.0, ArticleFromJournal
|Publication indicators||: 2015 = 2.134 (2) - 2015=2.149 (5); = 9|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.