Future of protease activity assays

Magdalena Wysocka , Adam Lesner

Abstract

Proteolysis is doubtlessly the most widespread mechanism of biological regulation. By controlling protein synthesis, turnover and activity, it is involved in fundamental physiological processes including apoptosis, cell differentiation, growth and signaling, fertilization, immune response, blood coagulation and digestion. Yet, uncontrolled proteolysis can be harmful for organisms, causing - amongst others - such diseases as cancer, emphysema, inflammation, and neurodegenerative, immunological, and cardiovascular disorders. This paper briefly describes recent advances in the development of methodological design to follow up protease activity. Novel methods of protease sensing are described and evaluated. A variety of fluorescent reporter molecules including nanoparticles, and rare metal chelates are also characterized.
Author Magdalena Wysocka (FCh / DBCh / LMCh)
Magdalena Wysocka,,
- Laboratory of Medical Chemistry
, Adam Lesner (FCh / DMB / LBA)
Adam Lesner,,
- Laboratory of Biochemical Analytics
Journal seriesCurrent Pharmaceutical Design, ISSN 1381-6128, (A 30 pkt)
Issue year2013
Vol19
No6
Pages1062-1067
Publication size in sheets0.5
Keywords in EnglishProtease, substrate, fluorescence, reporter molecules
DOIDOI:10.2174/1381612811319060008
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, ArticleFromJournal
Publication indicators WoS Citations = 10; WoS Impact Factor: 2013 = 3.288 (2) - 2013=3.555 (5)
Citation count*13 (2019-03-23)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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