Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)
Justyna Wróbel-Marek , Ewa Kurczyńska , Bartosz J. Płachno , Małgorzata Kozieradzka-Kiszkurno
AbstractMain conclusion Our study demonstrated that sym- plasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regula- tion of cell differentiation and plant development. Most recent studies concerning an analysis of symplasmic communication between seed compartments and the embryo have been predominantly performed on Ara- bidopsis thaliana . The results presented in this paper describe the analysis of symplasmic communication on the example of Sedum acre seeds, because the ultrastructure of the seed compartments and the embryo proper, including the PD, have already been described, and this species represents an embryonic type of development different to Arabidopsis . Moreover, in this species, an unusual elec- tron-dense dome associated with plasmodesmata on the border between the basal cell/chalazal suspensor cells and the basal cell/the endosperm has been described. This prompted the question as to whether these plasmodesmata are functional. Thus, the aim of this study was to describe the movement of symplasmic transport fluorochromes between different Sedum seed compartments, with partic- ular emphasis on the movement between the basal cell and the embryo proper and endosperm, to answer the following questions: (1) are seeds divided into symplasmic domains; (2) if so, are they stable or do they change with the development? The results have shown that symplasmic tracers movement: (a) from the external integument to internal integument is restricted; (b) from the basal cell to the other part of the embryo proper and from the basal cell to the endosperm is also restricted; (c) the embryo is a single symplasmic domain with respect to molecules of a molecular weight below 0.5 kDa.
|Journal series||Planta, ISSN 0032-0935, (A 35 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||embryogenesis, plasmodesmata, symplasmic communication, symplasmic domains|
|License||Other; published final; ; with publication|
|Score|| = 35.0, 20-12-2017, ArticleFromJournal|
= 40.0, 20-12-2017, ArticleFromJournal
|Publication indicators||: 2016 = 3.361 (2) - 2016=3.696 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.