Identification of symplasmic domains in the embryo and seed of Sedum acre L. (Crassulaceae)

Justyna Wróbel-Marek , Ewa Kurczyńska , Bartosz J. Płachno , Małgorzata Kozieradzka-Kiszkurno

Abstract

Main conclusion Our study demonstrated that sym- plasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regula- tion of cell differentiation and plant development. Most recent studies concerning an analysis of symplasmic communication between seed compartments and the embryo have been predominantly performed on Ara- bidopsis thaliana . The results presented in this paper describe the analysis of symplasmic communication on the example of Sedum acre seeds, because the ultrastructure of the seed compartments and the embryo proper, including the PD, have already been described, and this species represents an embryonic type of development different to Arabidopsis . Moreover, in this species, an unusual elec- tron-dense dome associated with plasmodesmata on the border between the basal cell/chalazal suspensor cells and the basal cell/the endosperm has been described. This prompted the question as to whether these plasmodesmata are functional. Thus, the aim of this study was to describe the movement of symplasmic transport fluorochromes between different Sedum seed compartments, with partic- ular emphasis on the movement between the basal cell and the embryo proper and endosperm, to answer the following questions: (1) are seeds divided into symplasmic domains; (2) if so, are they stable or do they change with the development? The results have shown that symplasmic tracers movement: (a) from the external integument to internal integument is restricted; (b) from the basal cell to the other part of the embryo proper and from the basal cell to the endosperm is also restricted; (c) the embryo is a single symplasmic domain with respect to molecules of a molecular weight below 0.5 kDa.
Author Justyna Wróbel-Marek
Justyna Wróbel-Marek,,
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, Ewa Kurczyńska
Ewa Kurczyńska,,
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, Bartosz J. Płachno
Bartosz J. Płachno,,
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, Małgorzata Kozieradzka-Kiszkurno KCiER
Małgorzata Kozieradzka-Kiszkurno,,
- Department of Plant Cytology and Embryology
Journal seriesPlanta, ISSN 0032-0935
Issue year2017
Vol245
No3
Pages491-505
Publication size in sheets0.7
Keywords in Englishembryogenesis, plasmodesmata, symplasmic communication, symplasmic domains
DOIDOI:10.1007/s00425-016-2619-y
URL http://link.springer.com/content/pdf/10.1007%2Fs00425-016-2619-y.pdf
Languageen angielski
LicenseOther; published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)40
ScoreMinisterial score = 35.0, 20-12-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 20-12-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.361 (2) - 2016=3.696 (5)
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