Floral anatomy, ultrastructure and chemical analysis in Dactylorhiza incarnata/maculata complex (Orchidaceae)

Aleksandra Naczk , Agnieszka Kowalkowska , Natalia Wiśniewska , Łukasz Haliński , Małgorzata Kapusta , Małgorzata Czerwicka

Abstract

Four Dactylorhiza taxa in the D. incarnata/maculata complex were examined to identify differences in floral traits, using micromorphological, histochemical and ultrastructural studies on spur and labellum structure. Flowers were collected at anthesis from four monospecific populations in Gdańsk, Pomerania, northern Poland. The flowers revealed secretory activity of the epidermis of the spur mouth (possible osmophores) and of the inner epidermis of spurs (possible nectar traces) in D. incarnata, D. maculata subsp. fuchsii and D. majalis subsp. majalis. The secretion was absent on papillae in D. maculata subsp. maculata. However, the presence of slightly developed micro-channels, vesicles in periplasmic space and plastoglobuli in plastids may be responsible for production of a meagre amount of fragrance or nectar. The examined Dactylorhiza taxa were also clearly separated by their scent profiles. The most abundant class of compounds is based on the common structure of 4-hydroxybenzene. The presence of vanillin precursors, such as 4-hydroxybenzaldehyde, which, like vanillin, are widely recognized to be important in attracting potential pollinators, was observed mainly in D. incarnata and D. majalis subsp. majalis. The scent compounds were characteristic for a particular species, which may be important for their floral isolation.
Author Aleksandra Naczk (FB / DME)
Aleksandra Naczk,,
- Department of Molecular Evolution
, Agnieszka Kowalkowska (FB / DPCE)
Agnieszka Kowalkowska,,
- Department of Plant Cytology and Embryology
, Natalia Wiśniewska (FB / DPCE)
Natalia Wiśniewska ,,
- Department of Plant Cytology and Embryology
, Łukasz Haliński (FCh / DEA / LANC)
Łukasz Haliński,,
- Laboratory of Analysis of Natural Compounds
, Małgorzata Kapusta (FB / DPCE)
Małgorzata Kapusta,,
- Department of Plant Cytology and Embryology
, Małgorzata Czerwicka (FCh / DBCh / LSB)
Małgorzata Czerwicka,,
- Laboratory of Structural Biochemistry
Journal seriesBotanical Journal of the Linnean Society, ISSN 0024-4074, (A 30 pkt)
Issue year2018
Vol187
No3
Pages512-536
Publication size in sheets1.2
Keywords in Englishfloral scent, food deception, GC-MS, micromorphology, ultrastructure
DOIDOI:10.1093/botlinnean/boy027
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 30.0, 19-06-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 19-06-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.277 (2) - 2016=2.426 (5)
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